概述

  • 产品名称Anti-Cortactin抗体
    参阅全部 Cortactin 一抗
  • 描述
    兔多克隆抗体to Cortactin
  • 经测试应用适用于: WB, ICC/IF, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human
  • 免疫原

    Synthetic peptide corresponding to the N-terminal region of human cortactin (amino acids 18-35) conjugated to KLH.

  • 阳性对照
    • Human epidermoid carcinoma, A431 cell line for Western blotting, the mouse fibroblasts NIH3T3 cell line for immunofluorescence.
  • 常规说明If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 15mM sodium azide.
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab11065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 65-75 kDa. This is determined using a rat brain cytosol extract, a whole extract of chicken embryo fibroblasts, or a whole cell extract of the human epidermoid carcinoma A431 cell line.
ICC/IF Use at an assay dependent dilution. PubMed: 20689769
IP Use at an assay dependent dilution. PubMed: 16971514

靶标

Anti-Cortactin antibody (ab11065)参考文献

This product has been referenced in:
  • Ponceau A  et al. aII-spectrin regulates invadosome stability and extracellular matrix degradation. PLoS One 10:e0120781 (2015). Read more (PubMed: 25830635) »
  • Spillane M  et al. Nerve growth factor-induced formation of axonal filopodia and collateral branches involves the intra-axonal synthesis of regulators of the actin-nucleating Arp2/3 complex. J Neurosci 32:17671-89 (2012). Read more (PubMed: 23223289) »

See all 4 Publications for this product

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