Anti-Coronin 3抗体(ab77203)

概述

  • 产品名称Anti-Coronin 3抗体
    参阅全部 Coronin 3 一抗
  • 描述
    小鼠单克隆抗体to Coronin 3
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow
  • 免疫原

    Recombinant fragment: EEWFEGKNAD PILISLKHGY IPGKNRDLKV VKKNILDSKP TANKKCDLIS IPKKTTDTAS VQNEAKLDEI LKEIKSIKDT ICNQDERISK LEQQMAKIAA, corresponding to amino acids 375-474 of Human Coronin 3 (NP_055140) with proprietary tag.

  • 阳性对照
    • Hela Nuclear Extract. Recombinant tagged Human Coronin 3 fragment.

性能

应用

Our Abpromise guarantee covers the use of ab77203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 53 kDa.
ELISA Use at an assay dependent dilution.

靶标

Anti-Coronin 3 antibody 图像

  • Anti-Coronin 3 antibody (ab77203) at 1 µg/ml + Hela Nuclear extract at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
  • Anti-Coronin 3 antibody (ab77203) at 1 µg/ml + Recombinant tagged Human Coronin 3 fragment at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 53 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

Anti-Coronin 3 antibody (ab77203)参考文献

ab77203 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (gastric cancer cell lines and Hela cell line)
Loading amount 25 µg
Specification gastric cancer cell lines and Hela cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Gui Ren

Verified customer

提交于 Feb 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"