概述

  • 产品名称Anti-Coronin 1a抗体
    参阅全部 Coronin 1a 一抗
  • 描述
    兔多克隆抗体to Coronin 1a
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • 免疫原

    Synthetic peptide (Human) corresponding to a region between residues 175 and 225 of Coronin 1a

  • 阳性对照
    • Jurkat whole cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab72212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes.
  • 组织特异性Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut.
  • 序列相似性Belongs to the WD repeat coronin family.
    Contains 7 WD repeats.
  • 细胞定位Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle > phagosome membrane. In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Actin binding protein antibody
    • CLABP antibody
    • Clipin A antibody
    • Clipin-A antibody
    • CLIPINA antibody
    • COR1A_HUMAN antibody
    • CORO1A antibody
    • Coronin 1 antibody
    • Coronin actin binding protein 1A antibody
    • Coronin like protein A antibody
    • Coronin like protein p57 antibody
    • Coronin-1A antibody
    • Coronin-like protein A antibody
    • Coronin-like protein p57 antibody
    • FLJ41407 antibody
    • HCORO1 antibody
    • MGC117380 antibody
    • OTTHUMP00000163017 antibody
    • p57 antibody
    • TACO antibody
    • Tryptophan aspartate containing coat protein antibody
    • Tryptophan aspartate-containing coat protein antibody
    see all

Anti-Coronin 1a antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling Coronin 1a with ab72212 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-Coronin 1a antibody (ab72212) at 0.04 µg/ml

    Lane 1 : Jurkat whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 51 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation analysis of  Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded). ab72212 used for IP at 3 µg/mg lysate and for WB at 1 µg/ml. Lane 1 - ab72212 IP. Lane 2 - control IgG.

Anti-Coronin 1a antibody (ab72212)参考文献

ab72212 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab72212.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"