Anti-CORO1B (phospho S2)抗体(ab119705)

概述

  • 产品名称Anti-CORO1B (phospho S2)抗体
    参阅全部 CORO1B 一抗
  • 描述
    兔多克隆抗体to CORO1B (phospho S2)
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Rabbit, Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phospho-peptide, coupled to carrier protein, corresponding to amino acids surrounding serine 2 of Human CORO1B.

  • 阳性对照
    • A431 and HelA cell lysateS, treated with Calyculin A; Rabbit spleen fibroblasts, treated with Calyculin A.
  • 常规说明Do not aliquot.

性能

应用

Our Abpromise guarantee covers the use of ab119705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 54 kDa.
ICC/IF 1/100.

靶标

  • 功能Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction.
  • 序列相似性Belongs to the WD repeat coronin family.
    Contains 5 WD repeats.
  • 翻译后修饰Phosphorylation by PKC on Ser-2 regulates the interaction with the Arp2/3 complex and cell motility in fibroblasts. Phosphorylation does not seem to affect subcellular location.
  • 细胞定位Cytoplasm > cytoskeleton. Localized to the leading edge in fibroblasts, as well as weakly along actin stress fibers.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COR1B_HUMAN antibody
    • CORO 1B antibody
    • CORO1B antibody
    • Coronin 1B antibody
    • Coronin 2 antibody
    • Coronin actin binding protein 1B antibody
    • Coronin-1B antibody
    • Coronin-2 antibody
    • Coronin1B antibody
    • Coronin2 antibody
    • DKFZP762I166 antibody
    • OTTHUMP00000236823 antibody
    see all

Anti-CORO1B (phospho S2) antibody 图像

  • Lanes 1 - 2 : Anti-CORO1B (C terminal) at 1/1000 dilution
    Lanes 3 - 4 : Anti-CORO1B (phospho S2) antibody (ab119705) at 1/1000 dilution

    Lane 1 : Human A431 cells treated with Calyculin A (100 nM) for 30 min
    Lane 2 : Human A431 cells treated with Calyculin A (100 nM) for 30 min before treatment with lambda phosphatase
    Lane 3 : Human A431 cells treated with Calyculin A (100 nM) for 30 min
    Lane 4 : Human A431 cells treated with Calyculin A (100 nM) for 30 min before treatment with lambda phosphatase


    Predicted band size : 54 kDa
  • ab119705 at 1/100 dilution staining CORO1B in Rabbit spleen fibroblasts treated with Calyculin A in the absence (left) or presence (right) of blocking peptide.
    Detection used an appropriate secondary antibody conjugated to Cy3.

Anti-CORO1B (phospho S2) antibody (ab119705)参考文献

ab119705 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119705.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"