概述

  • 产品名称Anti-CoREST3 / RCOR3抗体
    参阅全部 CoREST3 / RCOR3 一抗
  • 描述
    兔多克隆抗体to CoREST3 / RCOR3
  • 经测试应用适用于: WB, IP, IHC-P, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 445 and 495 of human CoREST3/ RCOR3 (NP_060724.1), (and between residues 503-553 NP_001129695.1.).

  • 阳性对照
    • HeLa, 293T and NIH3T3 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab76921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500.
ICC 1/50 - 1/250.

Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

ICC/IF 1/50 - 1/250.

靶标

  • 相关性The CoREST3 gene encodes a functional corepressor required for regulation of neural specific gene expression. REST (RE1 silencing transcription factor 1) can mediate extraneuronal restriction by imposing either active repression via histone deacetylase recruitment or long term gene silencing using a distinct functional complex. Silencing of neuronal specific genes requires the recruitment of an associated corepressor, CoREST, that serves as a functional molecular beacon for the recruitment of molecular machinery that imposes silencing across a chromosomal interval, including transcriptional units that do not themselves contain REST response elements.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • 4921514E24Rik antibody
    • C730034D20Rik antibody
    • E130101E15Rik antibody
    • FLJ10876 antibody
    • FLJ16298 antibody
    • KIAA1343 antibody
    • MGC28186 antibody
    • OTTHUMP00000035053 antibody
    • RCOR3 antibody
    • REST corepressor 3 antibody
    • RP11-318L16.1 antibody
    see all

Anti-CoREST3 / RCOR3 antibody 图像

  • Immunocytochemistry/Immunofluorescence analysis of NBF-fixed HeLa cells labelling CoREST3 / RCOR3 with ab76921 at 1/80 (2.5µg/ml). A DyLight® 488-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.
  • All lanes : Anti-CoREST3 / RCOR3 antibody (ab76921) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 66 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 second
  • Detection of CoREST3 / RCOR3 by Western Blot of Immunprecipitate.
    ab76921 at 1µg/ml staining CoREST3 / RCOR3 in HeLa whole cell lysate immunoprecipitated using ab76921 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-CoREST3 / RCOR3 antibody (ab76921)参考文献

This product has been referenced in:
  • Sáez JE  et al. Decreased Expression of CoREST1 and CoREST2 Together with LSD1 and HDAC1/2 during Neuronal Differentiation. PLoS One 10:e0131760 (2015). Read more (PubMed: 26111147) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab76921.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"