概述

  • 产品名称
    Anti-CoREST2 / RCOR2抗体
    参阅全部 CoREST2 / RCOR2 一抗
  • 描述
    兔多克隆抗体to CoREST2 / RCOR2
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human CoREST2/ RCOR2.

    (Peptide available as ab37307.)

  • 阳性对照
    • IHC-P: FFPE human testis tissue secions.

性能

应用

Our Abpromise guarantee covers the use of ab37113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 58 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

图片

  • IHC image of CoREST/RCOR2 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37113, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-CoREST2 / RCOR2 antibody (ab37113) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,260 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Anti-CoREST2 / RCOR2 antibody (ab37113) at 1/1000 dilution + MCF7 cells

    Secondary
    HRP conjugated donkey anti-rabbit at 1/2000 dilution.
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous abreview.

    See Abreview

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Sample
Mouse Cell lysate - whole cell (HEK293T overexpressing Flag-tagged mouse RCOR2 and)
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Loading amount
60 µg
Specification
HEK293T overexpressing Flag-tagged mouse RCOR2 and
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Nov 23 2015

Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (HEK293T overexpressing Flag-tagged mouse RCOR2)
Total protein in input
50 µg
Immuno-precipitation step
Other - Prot G Dynabeads
Specification
HEK293T overexpressing Flag-tagged mouse RCOR2
Username

Abcam user community

Verified customer

提交于 Nov 23 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7)
Loading amount
100000 cells
Specification
MCF7
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Jul 02 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册