概述

  • 产品名称Anti-COPS8抗体
    参阅全部 COPS8 一抗
  • 描述
    小鼠单克隆抗体to COPS8
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length human COPS8 protein (AAH03090) with a 26kDa tag.

  • 阳性对照
    • COPS8 transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab77462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 23 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
  • 序列相似性Belongs to the CSN8 family.
    Contains 1 PCI domain.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COP9 homolog antibody
    • COP9 signalosome complex subunit 8 antibody
    • COP9 signalosome subunit 8 antibody
    • Cops8 antibody
    • CSN8 antibody
    • CSN8_HUMAN antibody
    • hCOP9 antibody
    • JAB1 containing signalosome subunit 8 antibody
    • JAB1-containing signalosome subunit 8 antibody
    • SGN8 antibody
    • Signalosome subunit 8 antibody
    see all

Anti-COPS8 antibody 图像

  • Anti-COPS8 antibody (ab77462) at 1/500 dilution + immunogen at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 23 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-COPS8 antibody (ab77462) at 1/500 dilution

    Lane 1 : COPS8 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 23 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)

Anti-COPS8 antibody (ab77462)参考文献

ab77462 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77462.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"