概述

  • 产品名称Anti-COPS4抗体
    参阅全部 COPS4 一抗
  • 描述
    兔多克隆抗体to COPS4
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide, which represented a c-terminal domain of human COP9 signalosome complex subunit 4 (LocusLink ID 51138).

  • 阳性对照
    • HEK 293T cells.

性能

应用

Our Abpromise guarantee covers the use of ab12322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/5000 - 1/25000. Predicted molecular weight: 49 kDa.

靶标

  • 功能Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
  • 序列相似性Belongs to the CSN4 family.
    Contains 1 PCI domain.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CH4 antibody
    • COP9 (constitutive photomorphogenic) homolog subunit 4 antibody
    • COP9 complex homolog subunit 4 antibody
    • COP9 complex S4 antibody
    • COP9 constitutive photomorphogenic homolog subunit 4 (Arabidopsis) antibody
    • COP9 constitutive photomorphogenic homolog subunit 4 antibody
    • COP9 signalosome complex subunit 4 antibody
    • COP9 signalosome subunit 4 antibody
    • COPS4 antibody
    • CSN complex subunit 4 antibody
    • CSN4 antibody
    • CSN4 SCHPO antibody
    • CSN4_HUMAN antibody
    • DCH4 antibody
    • JAB1 containing signalosome subunit 4 antibody
    • JAB1-containing signalosome subunit 4 antibody
    • MGC94896 antibody
    • SGN4 antibody
    • Signalosome subunit 4 antibody
    see all

Anti-COPS4 antibody 图像



  • Predicted band size : 49 kDa


    Detection of Human CSN4 by Western Blot.
    Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN4 expression construct (T).
    Antibody: Affinity purified rabbit anti-CSN4 antibody BL1061 used at the indicated concentrations.
    Detection: Chemiluminescence with a 10 second exposure.
  • ICC/IF image of ab12322 stained MCF7 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12322, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-COPS4 antibody (ab12322)参考文献

This product has been referenced in:
  • Füzesi-Levi MG  et al. Dynamic regulation of the COP9 signalosome in response to DNA damage. Mol Cell Biol 34:1066-76 (2014). WB ; Human . Read more (PubMed: 24421388) »
  • Oh W  et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem 281:17457-65 (2006). Read more (PubMed: 16624822) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (amniotic fluid stem cells)
Loading amount 5 µg
Specification amniotic fluid stem cells
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"