- 数据表
- 具体的参考文献 (2)
- 实验方案
概述
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产品名称Anti-COPS3抗体
参阅全部 COPS3 一抗 -
描述兔多克隆抗体to COPS3
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宿主Rabbit
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经测试应用适用于: IHC-P, WBmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Platypus
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免疫原
Immunogen was a synthetic peptide, which represented a portion of human COP9 signalosome complex subunit 3 encoded within exon 10 (LocusLink ID 8533).
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阳性对照
- HEK 293T cells.
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常规说明Centrifuge tube to remove product from lid.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8 -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明Purified using the peptide immobilized on solid support.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab12321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/5000 - 1/25000. Predicted molecular weight: 51 kDa. |
靶标
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功能Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
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组织特异性Widely expressed. Expressed at high level in heart and skeletal muscle.
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序列相似性Belongs to the CSN3 family.
Contains 1 PCI domain. -
细胞定位Cytoplasm. Nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 416504 Chicken
- Entrez Gene: 507932 Cow
- Entrez Gene: 479528 Dog
- Entrez Gene: 8533 Human
- Entrez Gene: 26572 Mouse
- Entrez Gene: 100171418 Orangutan
- Entrez Gene: 287367 Rat
- Entrez Gene: 379813 Xenopus laevis
see all -
别名
- Constitutive photomorphogenic homolog subunit 3 antibody
- COP 9 (constitutive photomorphogenic) subunit 3 antibody
- COP 9 complex homolog subunit 3 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPS3 antibody (ab12321)Immunohisotchemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling COPS3 with ab12321 at 1/1000 (1µg/ml). Detection: DAB. -
Western blot - Anti-COPS3 antibody (ab12321)Detection of Human CSN3 by Western Blot. Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN3 expression construct (T). Antibody: Affinity purified rabbit anti-CSN3 antibody BL1059 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.
Detection of Human CSN3 by Western Blot.
Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN3 expression construct (T).
Antibody: Affinity purified rabbit anti-CSN3 antibody BL1059 used at the indicated concentrations.
Detection: Chemiluminescence with a 10 second exposure.
文献
This product has been referenced in:
- Olma MH et al. An interaction network of the mammalian COP9 signalosome identifies Dda1 as a core subunit of multiple Cul4-based E3 ligases. J Cell Sci 122:1035-44 (2009). WB ; Human . Read more (PubMed: 19295130) »
- Oh W et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem 281:17457-65 (2006). Read more (PubMed: 16624822) »
客户评价及客户问答
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