概述

  • 产品名称Anti-COPS3抗体
    参阅全部 COPS3 一抗
  • 描述
    兔多克隆抗体to COPS3
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human COP9 signalosome complex subunit 3 encoded within exon 10 (LocusLink ID 8533).

  • 阳性对照
    • HEK 293T cells.
  • 常规说明Centrifuge tube to remove product from lid.

性能

应用

Our Abpromise guarantee covers the use of ab12321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000 - 1/25000. Predicted molecular weight: 51 kDa.

靶标

  • 功能Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
  • 组织特异性Widely expressed. Expressed at high level in heart and skeletal muscle.
  • 序列相似性Belongs to the CSN3 family.
    Contains 1 PCI domain.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Constitutive photomorphogenic homolog subunit 3 antibody
    • COP 9 (constitutive photomorphogenic) subunit 3 antibody
    • COP 9 complex homolog subunit 3 antibody
    • COP 9 complex S3 antibody
    • COP 9 complex subunit 3 antibody
    • COP 9 constitutive photomorphogenic homolog subunit 3 antibody
    • COP 9 homolog antibody
    • COP 9 signalosome complex subunit 3 antibody
    • COP 9 signalosome subunit 3 antibody
    • COP 9 subunit 3 antibody
    • COP9 (constitutive photomorphogenic) subunit 3 antibody
    • COP9 complex homolog subunit 3 antibody
    • COP9 complex S3 antibody
    • COP9 complex subunit 3 antibody
    • COP9 constitutive photomorphogenic homolog subunit 3 antibody
    • COP9 homolog antibody
    • COP9 signalosome complex subunit 3 antibody
    • COP9 signalosome subunit 3 antibody
    • COP9 subunit 3 antibody
    • COPS 3 antibody
    • cops3 antibody
    • CSN 3 antibody
    • CSN3 antibody
    • CSN3_HUMAN antibody
    • JAB 1 containing signalosome subunit 3 antibody
    • JAB1 containing signalosome subunit 3 antibody
    • JAB1-containing signalosome subunit 3 antibody
    • SGN 3 antibody
    • SGN3 antibody
    • Signalosome subunit 3 antibody
    see all

Anti-COPS3 antibody 图像

  • Immunohisotchemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling COPS3 with ab12321 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 51 kDa

    Detection of Human CSN3 by Western Blot. Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN3 expression construct (T). Antibody: Affinity purified rabbit anti-CSN3 antibody BL1059 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.

    Detection of Human CSN3 by Western Blot.
    Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN3 expression construct (T).
    Antibody: Affinity purified rabbit anti-CSN3 antibody BL1059 used at the indicated concentrations.
    Detection: Chemiluminescence with a 10 second exposure.

Anti-COPS3 antibody (ab12321)参考文献

This product has been referenced in:
  • Olma MH  et al. An interaction network of the mammalian COP9 signalosome identifies Dda1 as a core subunit of multiple Cul4-based E3 ligases. J Cell Sci 122:1035-44 (2009). WB ; Human . Read more (PubMed: 19295130) »
  • Oh W  et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem 281:17457-65 (2006). Read more (PubMed: 16624822) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab12321.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"