Immunogen was a synthetic peptide, which represented a portion of human COP9 signalosome complex subunit 3 encoded within exon 10 (LocusLink ID 8533).
Our Abpromise guarantee covers the use of ab12321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
WB | 1/5000 - 1/25000. Predicted molecular weight: 51 kDa. |
Detection of Human CSN3 by Western Blot. Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN3 expression construct (T). Antibody: Affinity purified rabbit anti-CSN3 antibody BL1059 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.
Detection of Human CSN3 by Western Blot.
Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN3 expression construct (T).
Antibody: Affinity purified rabbit anti-CSN3 antibody BL1059 used at the indicated concentrations.
Detection: Chemiluminescence with a 10 second exposure.
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