Anti-COPS3抗体(ab113365)

概述

  • 产品名称Anti-COPS3抗体
    参阅全部 COPS3 一抗
  • 描述
    山羊多克隆抗体to COPS3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog, Xenopus laevis, Zebrafish, Cynomolgus Monkey, Xenopus tropicalis
  • 免疫原

    Synthetic peptide:

    C-SLYKKNIQRLT

    , corresponding to internal sequence amino acids 301-311 of Human COPS3 (NP_003644.2).

  • 阳性对照
    • HEK293 lysate overexpressing Human COPS3 with DYKDDDDK tag.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab113365 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab113365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).

靶标

  • 功能Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
  • 组织特异性Widely expressed. Expressed at high level in heart and skeletal muscle.
  • 序列相似性Belongs to the CSN3 family.
    Contains 1 PCI domain.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Constitutive photomorphogenic homolog subunit 3 antibody
    • COP 9 (constitutive photomorphogenic) subunit 3 antibody
    • COP 9 complex homolog subunit 3 antibody
    • COP 9 complex S3 antibody
    • COP 9 complex subunit 3 antibody
    • COP 9 constitutive photomorphogenic homolog subunit 3 antibody
    • COP 9 homolog antibody
    • COP 9 signalosome complex subunit 3 antibody
    • COP 9 signalosome subunit 3 antibody
    • COP 9 subunit 3 antibody
    • COP9 (constitutive photomorphogenic) subunit 3 antibody
    • COP9 complex homolog subunit 3 antibody
    • COP9 complex S3 antibody
    • COP9 complex subunit 3 antibody
    • COP9 constitutive photomorphogenic homolog subunit 3 antibody
    • COP9 homolog antibody
    • COP9 signalosome complex subunit 3 antibody
    • COP9 signalosome subunit 3 antibody
    • COP9 subunit 3 antibody
    • COPS 3 antibody
    • cops3 antibody
    • CSN 3 antibody
    • CSN3 antibody
    • CSN3_HUMAN antibody
    • JAB 1 containing signalosome subunit 3 antibody
    • JAB1 containing signalosome subunit 3 antibody
    • JAB1-containing signalosome subunit 3 antibody
    • SGN 3 antibody
    • SGN3 antibody
    • Signalosome subunit 3 antibody
    see all

Anti-COPS3 antibody 图像

  • Lane 1 : Anti-COPS3 antibody (ab113365) at 1 µg/ml
    Lane 2 : Anti-COPS3 antibody (ab113365) at 1 mg/ml
    Lane 3 : anti-DYKDDDDK tag at 1/5000 dilution

    Lane 1 : HEK293 lysate (in RIPA buffer) overexpressing Human COPS3 with DYKDDDDK tag
    Lane 2 : Mock transfected HEK293 lysate
    Lane 3 : HEK293 lysate (in RIPA buffer) overexpressing Human COPS3 with DYKDDDDK tag

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Predicted band size : 48 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

Anti-COPS3 antibody (ab113365)参考文献

ab113365 has not yet been referenced specifically in any publications.

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