概述

  • 产品名称Anti-COP1抗体
    参阅全部 COP1 一抗
  • 描述
    兔多克隆抗体to COP1
  • 经测试应用适用于: IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Pig, Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    A region between residues 100 and 150 of human COP1. (NP_071902.2)

  • 阳性对照
    • HeLa whole cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab70890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
  • 应用说明IP: Use at a concentration of 2 - 5 µg/ml.


    Is unsuitable for WB. (Use ab70889).



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.
    • 组织特异性Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.
    • 通路Protein modification; protein ubiquitination.
    • 序列相似性Belongs to the COP1 family.
      Contains 1 RING-type zinc finger.
      Contains 7 WD repeats.
    • 结构域The RING finger domain, in addition to its role in ubiquitination, functions as a structural scaffold to bring two clusters of positive-charged residues within spatial proximity to mimic a bipartite nuclear localization signal (NLS).
    • 细胞定位Nucleus speckle. Cytoplasm. In the nucleus, it forms nuclear speckles.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Constitutive photomorphogenesis protein 1 homolog antibody
      • COP antibody
      • E3 ubiquitin-protein ligase RFWD2 antibody
      • hCOP1 antibody
      • Rfwd2 antibody
      • RFWD2_HUMAN antibody
      • ring finger and WD repeat domain 2 antibody
      • RING finger and WD repeat domain protein 2 antibody
      • RING finger protein 200 antibody
      see all

    Anti-COP1 antibody 图像

    • HeLa whole cell lysate (1 mg for IP, 20% of IP loaded).
      Antibodies:
      Lane 1: ab70889 at 3µg/ml for IP.
      Lane 2: ab70890 at 3µg/ml for IP.
      Lane 3: ab70891 at 3µg/ml for IP.
      Lane 4: IgG control
      Subsequent Western Blotting was carried out using ab70889 at 1µg/ml.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    Anti-COP1 antibody (ab70890)参考文献

    ab70890 has not yet been referenced specifically in any publications.

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