概述

  • 产品名称Anti-COP1抗体
    参阅全部 COP1 一抗
  • 描述
    兔多克隆抗体to COP1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 301-350 (MSGLYSPVSE DSTVPQFEAP SPSHSSIIDS TEYSQPPGFS GTSQTKKQPW) of Mouse COP1 (NP_036061).

  • 阳性对照
    • Mouse thymus lysate.

性能

应用

Our Abpromise guarantee covers the use of ab116122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.
  • 组织特异性Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the COP1 family.
    Contains 1 RING-type zinc finger.
    Contains 7 WD repeats.
  • 结构域The RING finger domain, in addition to its role in ubiquitination, functions as a structural scaffold to bring two clusters of positive-charged residues within spatial proximity to mimic a bipartite nuclear localization signal (NLS).
  • 细胞定位Nucleus speckle. Cytoplasm. In the nucleus, it forms nuclear speckles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Constitutive photomorphogenesis protein 1 homolog antibody
    • COP antibody
    • E3 ubiquitin-protein ligase RFWD2 antibody
    • hCOP1 antibody
    • Rfwd2 antibody
    • RFWD2_HUMAN antibody
    • ring finger and WD repeat domain 2 antibody
    • RING finger and WD repeat domain protein 2 antibody
    • RING finger protein 200 antibody
    see all

Anti-COP1 antibody 图像

  • Anti-COP1 antibody (ab116122) at 1 µg/ml + Mouse thymus lysate at 10 µg

    Predicted band size : 80 kDa

Anti-COP1 antibody (ab116122)参考文献

ab116122 has not yet been referenced specifically in any publications.

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