Anti-Contactin 1抗体(ab66265)

概述

  • 产品名称
    Anti-Contactin 1抗体
    参阅全部 Contactin 1 一抗
  • 描述
    兔多克隆抗体to Contactin 1
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Mouse Contactin 1.

    (Peptide available as ab74597.)

  • 阳性对照
    • This antibody gave a positive signal in Human, Mouse and Rat Brain Tissue Lysates.

性能

应用

Our Abpromise guarantee covers the use of ab66265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 113 kDa).

靶标

  • 功能
    Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.
  • 组织特异性
    Strongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression in lung, pancreas, kidney and skeletal muscle.
  • 疾病相关
    Defects in CNTN1 are the cause of Compton-North congenital myopathy (CNCM) [MIM:612540]. CNCM is a familial lethal form of congenital onset muscle weakness, inherited in an autosomal-recessive fashion and characterized by a secondary loss of beta2-syntrophin and alpha-dystrobrevin from the muscle sarcolemma, central nervous system involvement, and fetal akinesia.
  • 序列相似性
    Belongs to the immunoglobulin superfamily. Contactin family.
    Contains 4 fibronectin type-III domains.
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CNTN 1 antibody
    • CNTN antibody
    • Cntn1 antibody
    • CNTN1_HUMAN antibody
    • Contactin-1 antibody
    • Contactin1 antibody
    • F3 antibody
    • F3cam antibody
    • Glycoprotein gp135 antibody
    • gp 135 antibody
    • GP135 antibody
    • MYPCN antibody
    • Neural cell surface protein F3 antibody
    see all

图片

  • All lanes : Anti-Contactin 1 antibody (ab66265) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 113 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes
  • ICC/IF image of ab66265 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66265, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

ab66265 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Human Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 27 2015

Application
Western blot
Loading amount
75 µg
Gel Running Conditions
Reduced Denaturing (8)
Sample
Mouse Tissue lysate - whole (Brain tissue)
Specification
Brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 20 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册