Anti-Contactin 1抗体(ab66265)


  • 产品名称Anti-Contactin 1抗体
    参阅全部 Contactin 1 一抗
  • 描述
    兔多克隆抗体to Contactin 1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Mouse Contactin 1.

    (Peptide available as ab74597.)

  • 阳性对照
    • This antibody gave a positive signal in Human, Mouse and Rat Brain Tissue Lysates.



Our Abpromise guarantee covers the use of ab66265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 113 kDa).


  • 功能Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.
  • 组织特异性Strongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression in lung, pancreas, kidney and skeletal muscle.
  • 疾病相关Defects in CNTN1 are the cause of Compton-North congenital myopathy (CNCM) [MIM:612540]. CNCM is a familial lethal form of congenital onset muscle weakness, inherited in an autosomal-recessive fashion and characterized by a secondary loss of beta2-syntrophin and alpha-dystrobrevin from the muscle sarcolemma, central nervous system involvement, and fetal akinesia.
  • 序列相似性Belongs to the immunoglobulin superfamily. Contactin family.
    Contains 4 fibronectin type-III domains.
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CNTN 1 antibody
    • CNTN antibody
    • Cntn1 antibody
    • CNTN1_HUMAN antibody
    • Contactin-1 antibody
    • Contactin1 antibody
    • F3 antibody
    • F3cam antibody
    • Glycoprotein gp135 antibody
    • gp 135 antibody
    • GP135 antibody
    • MYPCN antibody
    • Neural cell surface protein F3 antibody
    see all

Anti-Contactin 1 antibody 图像

  • All lanes : Anti-Contactin 1 antibody (ab66265) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 113 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)

    Exposure time : 8 minutes
  • ICC/IF image of ab66265 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66265, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Contactin 1 antibody (ab66265)参考文献

ab66265 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (brain)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Specification brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

提交于 May 27 2015

Application Western blot
Loading amount 75 µg
Gel Running Conditions Reduced Denaturing (8)
Sample Mouse Tissue lysate - whole (Brain tissue)
Specification Brain tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Nov 20 2014