Anti-Connexin 45 / GJA7抗体(ab70365)

概述

  • 产品名称Anti-Connexin 45 / GJA7抗体
    参阅全部 Connexin 45 / GJA7 一抗
  • 描述
    兔多克隆抗体to Connexin 45 / GJA7
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Connexin 45/ GJA7.

    (Peptide available as ab91444.)

  • 阳性对照
    • This antibody gave a positive signal in Rat Lung Tissue Lysate and in the following whole cell lysates: SHSY5Y; SKNBE; SKNSH.

性能

应用

Our Abpromise guarantee covers the use of ab70365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 45 kDa).

靶标

Anti-Connexin 45 / GJA7 antibody 图像

  • All lanes : Anti-Connexin 45 / GJA7 antibody (ab70365) at 1 µg/ml

    Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa,68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • Anti-Connexin 45 / GJA7 antibody (ab70365) at 1 µg/ml + Lung (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 45 kDa


    Exposure time : 20 minutes

Anti-Connexin 45 / GJA7 antibody (ab70365)参考文献

ab70365 has not yet been referenced specifically in any publications.

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