Anti-Connexin 45 / GJA7抗体[5B9.2] (ab78408)

概述

  • 产品名称
    Anti-Connexin 45 / GJA7抗体[5B9.2]
    参阅全部 Connexin 45 / GJA7 一抗
  • 描述
    小鼠单克隆抗体[5B9.2] to Connexin 45 / GJA7
  • 经测试应用
    适用于: Flow Cyt, WB, ICCmore details
  • 种属反应性
    与反应: Rat, Rabbit, Human
    预测可用于: Mouse, Cow, Dog, Pig, Chinese hamster
  • 免疫原

    Synthetic peptide:

    QAYSHQNNPHGPRE

    conjugated to KLH, corresponding to amino acids 354-367 of Human Connexin 45/ GJA7

  • 表位
    Near C terminal, cytoplasmic.
  • 阳性对照
    • Connexin 45 / GJA7 transfected rat C6 cells.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

性能

应用

Our Abpromise guarantee covers the use of ab78408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC Use at an assay dependent concentration.

靶标

图片

  • ab78408 staining Connexin 45 / GJA7 transfected rat C6 cells by Immunocytochemistry.
  • Overlay histogram showing HeLa cells stained with ab78408 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78408, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (muscle)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
30 µg
Specification
muscle
Blocking step
Blocking buffer for fluorescent western blocking as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
Username

Miss. Nathalie Da Silva

Verified customer

提交于 Jul 13 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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