Anti-Connexin 43 / GJA1抗体- Intercellular Junction Marker (ab63851)

概述

  • 产品名称
    Anti-Connexin 43 / GJA1抗体- Intercellular Junction Marker
    参阅全部 Connexin 43 / GJA1 一抗
  • 描述
    兔多克隆抗体to Connexin 43 / GJA1 - Intercellular Junction Marker
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human, Nile tilapia
    预测可用于: Mouse, Rat, Rabbit, Chicken, Cow, Pig, Xenopus laevis, Non human primates, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Connexin 43/ GJA1.

    (Peptide available as ab92658.)

  • 阳性对照
    • This antibody gave a positive signal in HeLa and MCF7 Whole Cell Lysates.

性能

应用

Our Abpromise guarantee covers the use of ab63851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能
    One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
  • 组织特异性
    Expressed in the heart and fetal cochlea.
  • 疾病相关
    Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
    Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
    Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
    Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
    Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases.
  • 序列相似性
    Belongs to the connexin family. Alpha-type (group II) subfamily.
  • 细胞定位
    Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Connexin 43 antibody
    • Connexin-43 antibody
    • Cx 43 antibody
    • Cx43 antibody
    • CXA1_HUMAN antibody
    • DFNB38 antibody
    • Gap junction 43 kDa heart protein antibody
    • Gap junction alpha-1 protein antibody
    • Gap junction protein alpha 1 43kDa (connexin 43) antibody
    • Gap junction protein alpha 1 43kDa antibody
    • Gap junction protein alpha like antibody
    • GJA 1 antibody
    • Gja1 antibody
    • GJAL antibody
    • ODD antibody
    • ODDD antibody
    • ODOD antibody
    • SDTY3 antibody
    see all

图片

  • All lanes : Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab63851) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,55 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab63851 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63851, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, and HepG2 cells at 5µg/ml.

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (MCF7 cells)
Specification
MCF7 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 07 2015

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Non-reduced Denaturing (Upper-5% Lower 15%)
Sample
Nile tilapia (Oreochromis niloticus) Tissue lysate - whole (pituitary and heart)
Specification
pituitary and heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 24 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Nile tilapia (Oreochromis niloticus) Cell (Pituitary)
Specification
Pituitary
Permeabilization
Yes - triton-X 0.3%
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 24 2014

Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Loading amount
440 µg
Specification
Heart
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Ms. Branka Vulesevic

Verified customer

提交于 May 30 2012

Merci de votre intérêt pour les anti-connexine 43 d'Abcam.

Comme expliqué par téléphone,à notre connaissanceles anticorps http://www.abcam.com/ab62689 et http://www.abcam.com/ab63851 n’ont pas en...

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Thank you for your inquiry.

Unfortunately, the immunogen of ab1671 shows less than 60% homology with dog and therefore we do not expect a crpss-raction of this antibody. ab63851 does show a 93% homology with dog and would in general be eligab...

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