Anti-Connexin 43 / GJA1抗体(ab47441)
Key features and details
- Rabbit polyclonal to Connexin 43 / GJA1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Connexin 43 / GJA1抗体
参阅全部 Connexin 43 / GJA1 一抗 -
描述
兔多克隆抗体to Connexin 43 / GJA1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
The antiserum was produced against synthesized phosphopeptide derived from human Connexin 43 around the phosphorylation site of serine 367 (R-A-SP-S-R).
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阳性对照
- Extracts from K562 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47441于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
靶标
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功能
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. -
组织特异性
Expressed in the heart and fetal cochlea. -
疾病相关
Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases. -
序列相似性
Belongs to the connexin family. Alpha-type (group II) subfamily. -
细胞定位
Cell membrane. Cell junction > gap junction. - Information by UniProt
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数据库链接
- Entrez Gene: 2697 Human
- Entrez Gene: 14609 Mouse
- Entrez Gene: 24392 Rat
- Omim: 121014 Human
- SwissProt: P17302 Human
- SwissProt: P23242 Mouse
- SwissProt: P08050 Rat
- Unigene: 74471 Human
see all -
别名
- Connexin 43 antibody
- Connexin-43 antibody
- Cx 43 antibody
see all
图片
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All lanes : Anti-Connexin 43 / GJA1 antibody (ab47441) at 1/500 dilution
Lane 1 : Extracts from K562 cells, treated with PMA
(200ng/ml, 10min). No peptide.
Lane 2 : Extracts from K562 cells, treated with PMA
(200ng/ml, 10min). Synthetic peptide present.
Predicted band size: 43 kDa
Observed band size: 43 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab47441 尚未被引用在任何文献中。