Anti-Connexin 43 / GJA1抗体(ab11370)

概述

  • 产品名称Anti-Connexin 43 / GJA1抗体
    参阅全部 Connexin 43 / GJA1 一抗
  • 描述
    兔多克隆抗体to Connexin 43 / GJA1
  • 经测试应用适用于: ICC/IF, IHC-Fr, ICC, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Cow, Dog, Human, Pig, Monkey
  • 免疫原

    Synthetic peptide:

    KPSSRASSRASSRPRPDDLEI

    conjugated to KLH, corresponding to amino acids 362-382 of Human GJA1.

  • 阳性对照
    • Mouse brain tissue lysate, human testis, mouse heart tissue
  • 常规说明Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

性能

应用

Our Abpromise guarantee covers the use of ab11370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P 1/1000 - 1/5000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
WB 1/8000. Detects a band of approximately 43 kDa.

靶标

  • 功能One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
  • 组织特异性Expressed in the heart and fetal cochlea.
  • 疾病相关Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
    Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
    Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
    Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
    Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases.
  • 序列相似性Belongs to the connexin family. Alpha-type (group II) subfamily.
  • 细胞定位Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Connexin 43 antibody
    • Connexin-43 antibody
    • Cx 43 antibody
    • Cx43 antibody
    • CXA1_HUMAN antibody
    • DFNB38 antibody
    • Gap junction 43 kDa heart protein antibody
    • Gap junction alpha-1 protein antibody
    • Gap junction protein alpha 1 43kDa (connexin 43) antibody
    • Gap junction protein alpha 1 43kDa antibody
    • Gap junction protein alpha like antibody
    • GJA 1 antibody
    • Gja1 antibody
    • GJAL antibody
    • ODD antibody
    • ODDD antibody
    • ODOD antibody
    • SDTY3 antibody
    see all

Anti-Connexin 43 / GJA1 antibody 图像

  • Immunohistochemical detection of Connexin 43 / GJA1 using ab11370 (1/1000) on human testis sections (PFA-fixed paraffin-embedded sections). Antigen retrieval : Heat mediated - Buffer/Enzyme Used: Citric acid pH6, 1% BSA as blocking agent for 10 mins @ rt°C. Biotin labelled secondary antibody used at 1/200. Stained are clusters of Leydig cells in the interstitium and what should be Sertoli cells within the seminiferous tubules and the junction between (?). Sertoli cells and tubule "capsular" cells are intensely positive at their membrane interfaces (capsular interface positivity only evident in upper middle of image). Interestingly,smooth muscle of human colon and myometrium were completely negative when tested at the same time.

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  • All lanes : Anti-Connexin 43 / GJA1 antibody (ab11370) at 1/6000 dilution

    Lane 1 : Whole tissue lysate prepared from murine left ventricle at 5 µg
    Lane 2 : Whole tissue lysate prepared from murine left ventricle at 10 µg
    Lane 3 : Whole tissue lysate prepared from murine left ventricle at 20 µg
    Lane 4 : Whole tissue lysate prepared from murine left ventricle at 30 µg
    Lane 5 : Whole tissue lysate prepared from murine left ventricle at 40 µg

    Secondary
    HRP-conjugated donkey anti-rabbit polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Observed band size : 43 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    Image courtesy of an anonymous Abreview.

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  • ab11370 staining Connexin 43 / GJA1 in human pluripotent stem cell derived cardiomyocyte by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 5% serum for 15 minutes at room temperature. Samples were incubated with primary antibody (1/1000) for 16 hours at 4°C. An Alexa Fluor® 568-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    Clear antibody signal (yellow) at the interface of adjoining cells in a population of human embryonic stem cell derived cardiomyocytes (red = sarcomeric alpha actinin).

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  • ab11370 staining Connexin 43 / GJA1 in Mouse heart tissue by Immunohistochemistry (Frozen sections). The sections were fixed in Acetone prior to permeabilization with 1% Triton X-100 blocking with 10% horse serum for 1 hour at room temperature. The primary antibody was diluted 1/100 in 10% horse serum and incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/500. Results showed that Connexin 43 is expressed at cell-cell gap-junction.

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  • ab11370 staining Connexin 43/GJA1 in Pig heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/3000 in blocking buffer) for 2 hours at 22°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

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  •  Immunohistochemistal detection of Connexin 43/GJA1 with antibody ab11370 on PFA-fixed paraffin-embedded adult marmoset myocardium sections. Antigen retrieval step: Heat mediated in citric acid pH6. 1% BSA used for blocking (10 mins @ rt°C). Primary Antibody ab11370 used at 1/5000 for 2 hours. Secondary Antibody Name: anti-rabbit IgG biotin labelled (1/200). Submitted image has coloured arrowheads to indicate features: L/S myofibres show a classic intercalated disk positivity ( red). Fibres that appear to be oblique wrt orientation show an ovoid punctate positivity that I assume to be intercalated disks(black). This positivity is strongest in the circumference of each ovoid. There is also localised punctate positivity between adjacent myofibres (green).

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  • ab11370 staining Connexin 43/GJA1 in Dog Heart muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/6000 in blocking buffer) for 2 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

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  • Immunohistochemistical detection of  Connexin 43 / GJA1 using ab11370 on Formaldehyde-fixed paraffin-embedded mouse heart muscle sections. Antigen retrieval step: heat mediated using citric acid pH6. Blocking step: 1% BSA for 10 mins @ rt°C.  Primary antibody ab11370 incubated at 1/2000 for 2 hours.  Secondary antibody: anti rabbit IgG conjugated to biotin used at 1/200. Submitted image of cardiac myofibres in L/S shows clear, specific labelling of Intercalated discs which are rich in Gap junctions (Connexin 43 is a major component of Gap junctions). No other positivity is observed in mouse heart (the image is taken from a whole heart section). NB: in mouse tongue the ventral keratinising stratified squamous epithelium shows positivity in not only basal cells but also in the Prickle cell layer, although markedly reduced: data not shown but see here http://www.immunoportal.com/ for appropriate image.

Anti-Connexin 43 / GJA1 antibody (ab11370)参考文献

This product has been referenced in:
  • Zhang X  et al. Phenotypic transformation of smooth muscle cells from porcine coronary arteries is associated with connexin 43. Mol Med Rep 14:41-8 (2016). WB ; Pig . Read more (PubMed: 27175888) »
  • Yang Y  et al. The Increased Expression of Connexin and VEGF in Mouse Ovarian Tissue Vitrification by Follicle Stimulating Hormone. Biomed Res Int 2015:397264 (2015). IHC (PFA fixed) ; Mouse . Read more (PubMed: 26539488) »

See all 28 Publications for this product

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cat Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrated Buffer, pH6.0
Permeabilization No
Specification skin
Blocking step Casein in phosphate-buffered saline as blocking agent for 10 minute(s) · Concentration: 0.4% · Temperature: 37°C
Fixative Formaldehyde
Username

Mrs. Cinthya Borges

Verified customer

提交于 Nov 29 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Chicken Tissue sections (Heart muscle)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Heart muscle
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Nov 30 2015

Application Western blot
Sample Mouse Tissue lysate - whole (prefrontal cortex)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification prefrontal cortex
Blocking step LiCOR blocking buffer as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 50% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Oct 28 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Permeabilization Yes - saponin
Specification Human pluripotent stem cell derived cardiomyocyte
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 21 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Quail Tissue sections (Embryo)
Specification Embryo
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Feb 13 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Dog Tissue sections (Heart muscle)
Specification Heart muscle
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Nov 04 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Pig Tissue sections (Heart)
Specification Heart
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Nov 03 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8)
Sample Human Cell lysate - whole cell (myocyte)
Specification myocyte
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 15 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Cell (myocyte)
Specification myocyte
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 14 2013

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