Anti-Connexin 43 / GJA1抗体[4E6.2] (ab79010)

概述

  • 产品名称
    Anti-Connexin 43 / GJA1抗体[4E6.2]
    参阅全部 Connexin 43 / GJA1 一抗
  • 描述
    小鼠单克隆抗体[4E6.2] to Connexin 43 / GJA1
  • 特异性
    ab79010 is not expected to react with other connexins.
  • 经测试应用
    适用于: Flow Cyt, ICC/IF, WB, ELISA, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Dog, Human, Pig
    预测可用于: Sheep, Guinea pig, Cow, Chinese hamster
  • 免疫原

    Synthetic peptide:

    GPLSPSKDCGSPKYAYFNG

    , corresponding to amino acids 252-270 of Mouse Connexin 43/ GJA1

  • 阳性对照
    • Mouse and rat brain lysates. Mouse keratinocyte cells. Rat heart tissue.

性能

应用

Our Abpromise guarantee covers the use of ab79010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/50 - 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).Can be blocked with Connexin 43 / GJA1 peptide (ab171366).
ELISA Use at an assay dependent concentration.
IHC-Fr 1/50 - 1/250. Use Acetone/methanol fixed tissues (1:1, 20 minutes ice cold).

靶标

  • 功能
    One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
  • 组织特异性
    Expressed in the heart and fetal cochlea.
  • 疾病相关
    Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
    Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
    Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
    Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
    Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases.
  • 序列相似性
    Belongs to the connexin family. Alpha-type (group II) subfamily.
  • 细胞定位
    Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Connexin 43 antibody
    • Connexin-43 antibody
    • Cx 43 antibody
    • Cx43 antibody
    • CXA1_HUMAN antibody
    • DFNB38 antibody
    • Gap junction 43 kDa heart protein antibody
    • Gap junction alpha-1 protein antibody
    • Gap junction protein alpha 1 43kDa (connexin 43) antibody
    • Gap junction protein alpha 1 43kDa antibody
    • Gap junction protein alpha like antibody
    • GJA 1 antibody
    • Gja1 antibody
    • GJAL antibody
    • ODD antibody
    • ODDD antibody
    • ODOD antibody
    • SDTY3 antibody
    see all

图片

  • Immunohistochemistry of a human heart staining Connexin 43 / GJA1 with ab79010 at 1:500 dilution

  • Overlay histogram showing SH-SY5Y cells stained with ab79010 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79010, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemistry of a rat heart staining Connexin 43 / GJA1 with ab79010 at 1:500 dilution

  • Anti-Connexin 43 / GJA1 antibody [4E6.2] (ab79010) at 1/1000 dilution + Rat brain lysate at 10 µg

    Secondary
    Goat anti-mouse HRP

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • All lanes : Anti-Connexin 43 / GJA1 antibody [4E6.2] (ab79010) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Secondary
    Goat anti-mouse HRP

    Predicted band size : 43 kDa
    Observed band size : 43 kDa

文献

This product has been referenced in:
  • Zheng B  et al. Involvement of Rictor/mTORC2 in cardiomyocyte differentiation of mouse embryonic stem cells in vitro. Int J Biol Sci 13:110-121 (2017). Flow Cyt, ICC/IF ; Mouse . Read more (PubMed: 28123351) »
  • Li X  et al. Breakthrough Cancer Pain Is Associated with Spinal Gap Junction Activation via Regulation of Connexin 43 in a Mouse Model. Front Cell Neurosci 11:207 (2017). Read more (PubMed: 28769766) »

See all 9 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Sample
Chicken Tissue sections (Hindbrain section)
Specification
Hindbrain section
Permeabilization
Yes - PBS + 0.1% Tween20
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Corneal epithelium)
Specification
Corneal epithelium
Fixative
Aceton-Methanol
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Dec 10 2012

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