Anti-Connexin 40 / GJA5抗体(ab38580)

概述

  • 产品名称Anti-Connexin 40 / GJA5抗体
    参阅全部 Connexin 40 / GJA5 一抗
  • 描述
    兔多克隆抗体to Connexin 40 / GJA5
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human, Pig
  • 免疫原

    Synthetic peptide corresponding to Human Connexin 40/ GJA5 aa 102-134 (N terminal).
    Database link: P36382

  • 阳性对照
    • WB: Human placenta lysate. IHC-P: Human lung carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab38580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/10 - 1/50.
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).

靶标

  • 功能One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
  • 疾病相关Defects in GJA5 are a cause of familial atrial standstill (FAS) [MIM:108770]. Atrial standstill is an extremely rare arrhythmia, characterized by the absence of electrical and mechanical activity in the atria. Electrocardiographically, it is characterized by bradycardia, the absence of P waves, and a junctional narrow complex escape rhythm.
  • 序列相似性Belongs to the connexin family. Alpha-type (group II) subfamily.
  • 细胞定位Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Connexin 40 antibody
    • Connexin-40 antibody
    • Cx40 antibody
    • CXA5_HUMAN antibody
    • Gap junction alpha 5 protein antibody
    • Gap junction alpha-5 protein antibody
    • Gap junction protein alpha 5 40kD (connexin 40) antibody
    • Gap junction protein alpha 5 antibody
    • Gja5 antibody
    • MGC11185 antibody
    see all

Anti-Connexin 40 / GJA5 antibody 图像

  • Anti-Connexin 40 / GJA5 antibody (ab38580) + Human placenta lysate

    Predicted band size : 40 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Connexin 40 / GJA5 with ab38580. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.

  • ab38580 staining Connexin 40 / GJA5 in Mouse myocyte cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 10% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in serum) for 18 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit IgG monoclonal (1/1000) was used as the secondary antibody.

    See Abreview

Anti-Connexin 40 / GJA5 antibody (ab38580)参考文献

This product has been referenced in:
  • Zhang X  et al. Phenotypic transformation of smooth muscle cells from porcine coronary arteries is associated with connexin 43. Mol Med Rep 14:41-8 (2016). WB ; Pig . Read more (PubMed: 27175888) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step EnVision Flex Peroxidase-blocking reagent (ready-to-use - DAKO) as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample Cow Tissue sections (Fetal Liver)
Specification Fetal Liver
Permeabilization No
Fixative Formaldehyde
Username

Dr. Juliana Shimara Pires Ferrão

Verified customer

提交于 Nov 26 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Horse Tissue sections (aglandular stomach, epithelium)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Specification aglandular stomach, epithelium
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Methacarn
Username

Dr. F Hernandez-Blazquez

Verified customer

提交于 Mar 17 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Cell (myocyte)
Specification myocyte
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 13 2013

Thank you for contacting us.

Lysates of cardiomyocytes can be used as positive control.

http://198.170.104.138/ijp/2005/44-54.pdf

We unfortunaltey do not have these lysates. Please check other vendors websites.

For m...

Read More

Thank you for your email and for including the ICC images for your experiments with ab38580. Have you run a western blot to detect protein in your siRNA treated and untreated samples? Thanks!

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (heart)
Specification heart
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Universalblocking reagent from biogenex as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Mrs. Sina Huntscha

Verified customer

提交于 Jan 31 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"