Anti-Connexin 40 / GJA5抗体(ab16585)

概述

  • 产品名称Anti-Connexin 40 / GJA5抗体
    参阅全部 Connexin 40 / GJA5 一抗
  • 描述
    羊多克隆抗体to Connexin 40 / GJA5
  • 特异性Predicted not to cross react with Connexins 37, 43 or 45.
  • 经测试应用适用于: ICC, ICC/IFmore details
  • 种属反应性
    与反应: Rat
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to Diptheria toxoid carrier protein:

    SLVQGLTPPPDFNQCLK

    , corresponding to amino acids 254-270 of Rat GJA5.

  • 阳性对照
    • Cos-7 cells transfected with cDNA encoding GJA5. This antibody gave a positive result when used in the following methanol fixed cell lines: PC12

性能

应用

Our Abpromise guarantee covers the use of ab16585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/10000 - 1/50000.
ICC/IF Use at an assay dependent dilution.

靶标

  • 功能One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
  • 疾病相关Defects in GJA5 are a cause of familial atrial standstill (FAS) [MIM:108770]. Atrial standstill is an extremely rare arrhythmia, characterized by the absence of electrical and mechanical activity in the atria. Electrocardiographically, it is characterized by bradycardia, the absence of P waves, and a junctional narrow complex escape rhythm.
  • 序列相似性Belongs to the connexin family. Alpha-type (group II) subfamily.
  • 细胞定位Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Connexin 40 antibody
    • Connexin-40 antibody
    • Cx40 antibody
    • CXA5_HUMAN antibody
    • Gap junction alpha 5 protein antibody
    • Gap junction alpha-5 protein antibody
    • Gap junction protein alpha 5 40kD (connexin 40) antibody
    • Gap junction protein alpha 5 antibody
    • Gja5 antibody
    • MGC11185 antibody
    see all

Anti-Connexin 40 / GJA5 antibody 图像

  • ICC/IF image of ab16585 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab16585 at 1/200 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 donkey anti- Sheep (ab150177) IgG used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-Connexin 40 / GJA5 antibody (ab16585)参考文献

ab16585 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (atrium)
Specification atrium
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
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Abcam user community

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提交于 Apr 25 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (heart)
Specification heart
Fixative Paraformaldehyde
Permeabilization Yes - triton-X 100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Feb 23 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rabbit Tissue sections (right atrium, right ventricle and AV node and His)
Specification right atrium, right ventricle and AV node and His
Fixative Paraformaldehyde
Permeabilization Yes - triton-X 100
Username

Abcam user community

Verified customer

提交于 Aug 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"