Anti-Connexin 36 / GJA9抗体(ab86408)

概述

  • 产品名称Anti-Connexin 36 / GJA9抗体
    参阅全部 Connexin 36 / GJA9 一抗
  • 描述
    兔多克隆抗体to Connexin 36 / GJA9
  • 经测试应用适用于: IHC-FoFr, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 272 - 321 (

    LNHLGWRKIKLAVRGAQAKRKSIYEIRNKDLPRVSVPNFGRTQSSDSAYV

    ) of Human Connexin 36/GJA9 (NP_065711)

  • 阳性对照
    • Jurkat cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab86408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-Connexin 36 / GJA9 antibody 图像

  • Male mouse (P41) was anesthetized with isoflurane until they became non-responsive to tail pinch. Animal's thoracic cavity opened and it was transcardially perfused with 10 mL of ice cold PBS, then 10 mL of Ice cold 4 % PFA solution. Brain was removed and fixed overnight in 4% PFA. Next morning brain was rinsed 3X in PBS. Brain was immersed in agar and sliced at a thickness of 50uM. Brain slices were incubated overnight in a solution of PBST (0.2%) + 10% Normal GOAT serum + a monoclonal mouse anti-Parvalbumin antibody (1:2000)+ ab86408 (1ug/mL) and gently shaken overnight at 4°C. Samples were washed 6x in PBS (10 min each at RT) and exposed to secondary antibodies at 4°C: PBST (0.3%) + 10% normal goat serum + anti-mouse Alexa 488 (1:1000) + Anti-rabbit Alexa 555 (1:1000). Samples were washed 6x in PBST mounted. Images acquired with a confocal microscope at 63X.

  • Anti-Connexin 36 / GJA9 antibody (ab86408) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Gel concentration 12%

Anti-Connexin 36 / GJA9 antibody (ab86408)参考文献

This product has been referenced in:
  • Franco-Pérez J  et al. Sleep deprivation and sleep recovery modifies connexin36 and connexin43 protein levels in rat brain. Neuroreport 23:103-7 (2012). Read more (PubMed: 22158029) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Retina)
Gel Running Conditions Reduced Denaturing (NOVEX 4-20% Tris-Glycine)
Loading amount 20 µg
Treatment Contains untreated samples and retinas from mice exposed to high intensity light
Specification Retina
Blocking step Odyssey buffer / TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
Username

Marcus Hooper

Verified customer

提交于 May 17 2016

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ab86408 and ab48814 are fully characterized with human lysates in western blot. The image is published on the datasheet and the antibodies are fully guaranteed for human lysates.

Cow (bovine) is predict...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"