使用敲除细胞株进行验证RabMAb

Anti-COMT抗体[EPR6491(B)] (ab124813)

概述

  • 产品名称Anti-COMT抗体[EPR6491(B)]
    参阅全部 COMT 一抗
  • 描述
    兔单克隆抗体[EPR6491(B)] to COMT
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human COMT.

  • 阳性对照
    • Fetal Liver, A431 and MOLT4 cell lysates; A431 cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab124813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    • 功能Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
    • 组织特异性Brain, liver, placenta, lymphocytes and erythrocytes.
    • 序列相似性Belongs to the mammalian catechol-O-methyltransferase family.
    • 翻译后修饰The N-terminus is blocked.
    • 细胞定位Cytoplasm and Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Catechol O methyltransferase antibody
      • Catechol O-methyltransferase antibody
      • COMT antibody
      • COMT_HUMAN antibody
      • EC 2.1.1.6 antibody
      see all

    Anti-COMT antibody [EPR6491(B)] 图像



    • Predicted band size : 30 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: COMT knockout HAP1 whole cell lysate (20 µg)
      Lane 3: A431 whole cell lysate (20 µg)
      Lane 4: MOLT4 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab124813 observed at 28 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab124813 was shown to recognize COMT when COMT knockout samples were used, along with additional cross-reactive bands. Wild-type and COMT knockout samples were subjected to SDS-PAGE.  Ab124813 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-COMT antibody [EPR6491(B)] (ab124813) at 1/1000 dilution

      Lane 1 : Fetal Liver lysate
      Lane 2 : A431 cell lysate
      Lane 3 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique

      Predicted band size : 30 kDa
    • ab124813, at 1/10 dilution staining COMT in A431 cells, (Red) compared to a nonspecific negative control antibody (Green) by Flow Cytometry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-COMT antibody [EPR6491(B)] (ab124813)参考文献

    ab124813 has not yet been referenced specifically in any publications.

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