概述

  • 产品名称Anti-COMT抗体
    参阅全部 COMT 一抗
  • 描述
    山羊多克隆抗体to COMT
  • 特异性This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1).
  • 经测试应用适用于: WB, IHC-P, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-QDIIPQLKKKYDVD

    , corresponding to internal sequence amino acids 170-183 of Human COMT

  • 阳性对照
    • Human Testis lysates

性能

应用

Our Abpromise guarantee covers the use of ab51984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects an approximate 28kDa band and faint 25kDa band observed in human testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and 24.5kDa according to NP_009294.1).
IHC-P Use a concentration of 2 - 4 µg/ml.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Increasing amount of recombinant COMTcaptured by a rabbit antibody. ab51984 used as reporter.

靶标

Anti-COMT antibody 图像

  • Immunohistochemistry (paraffin-embedded sections) analysis of human cerebellum tissue labeling COMT with ab51984 at 4µg/ml. Microwaved antigen retrieval was performed using Tris/EDTA buffer pH 9. HRP staining.

  • Anti-COMT antibody (ab51984) at 0.03 µg/ml + Human Testis lysate in RIPA buffer at 35 µg

    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-COMT antibody (ab51984)参考文献

ab51984 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab51984.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"