Anti-Complex IV Immunocapture抗体[31E91B82G9] (ab109863)

概述

  • 产品名称Anti-Complex IV Immunocapture抗体[31E91B82G9]
  • 描述
    小鼠单克隆抗体[31E91B82G9] to Complex IV Immunocapture
  • 特异性100 µg ab109863 can capture at least 10 µg Complex IV (COX) from 1 mg solubilized Bovine heart mitochondria.
  • 经测试应用适用于: ICC/IF, IP, Flow Cytmore details
    不适用于: IHC-P or WB
  • 种属反应性
    与反应: Cow, Human
  • 免疫原

    Bovine Complex IV

  • 阳性对照
    • Bovine heart and Human mitochondria IF/ICC: HeLa cell line
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab109863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IP Use at 100-1 µg/mg of lysate.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for IHC-P or WB.
  • 靶标

    • 相关性Complex IV, also called cytochrome c oxidoreductase or cytochrome c oxidase, is a complex of 13 different subunits, three of which (I, II and III) are encoded on mitochondrial DNA and the remainder in the nuclear DNA. The complex contains two heme groups (a and a3) and two copper atoms as prosthetic groups. Genetic alterations of this enzyme complex are a common cause of OXPHOS diseases and the enzyme is altered in patients with Alzheimer’s disease. Also, there are reports of reduced amounts of this complex in hypoxic cancer cells.
    • 细胞定位Mitochondrial

    Anti-Complex IV Immunocapture antibody [31E91B82G9] 图像

    • ICC/IF image of ab109863 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109863, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Overlay histogram showing HepG2 cells stained with ab109863 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109863, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Staining of Complex IV Lane 1: 0.1 mg Human mitochondrial lysate Lane 2: 0.1 mg Bovine mitochondrial lysate Lane 3: 0.025 mg Human mitochondrial lysate Lane 4: 0.025 mg Bovine mitochondrial lysate Immunoprecipitated using ab109863 at 100 µg/mg lysate

    Anti-Complex IV Immunocapture antibody [31E91B82G9] (ab109863)参考文献

    This product has been referenced in:
    • Escobar-Alvarez S  et al. Inhibition of human peptide deformylase disrupts mitochondrial function. Mol Cell Biol 30:5099-109 (2010). Read more (PubMed: 20805355) »
    • Nadanaciva S  et al. Target identification of drug induced mitochondrial toxicity using immunocapture based OXPHOS activity assays. Toxicol In Vitro 21:902-11 (2007). Read more (PubMed: 17346924) »

    See all 2 Publications for this product

    Product Wall

    Vielen Dank für Ihre Email.

    Wir haben das Lot noch nicht rezeptionniert. Ich werde mich aber sogleich bei Ihnen melden, wenn wir es in unser Lager aufgenommen haben. (Ich habe einen täglichen Reminder in meinen Kalendar getan)
    ...

    Read More

    Vielen Dank für Ihren Anruf.

    Das Labor hat mir auch bestätigt, dass es zwischen x keinen Unterschied bei der Produktion gab. In der Tat kommen beide dieser Chargen von der gleichen ursprünglichen Produktion. Ich weiss daher lei...

    Read More

    Thank you for contacting us. Please click these links to find the suitable antibodies. Type the MS code in Abcam search box to get the Abcam catalogue number. Complex I http://www.mitosciences.com/complex_i.html Complex IV http://www.mi...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"