Complex IV人Specific Activity Microplate Assay试剂盒(ab109910)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture extracts, Tissue
概述
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产品名称
Complex IV人Specific Activity Microplate Assay试剂盒
参阅全部 Complex IV 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture extracts, Tissue -
检测类型
Enzyme activity -
种属反应性
与反应: Human -
产品概述
This rapid multiplexing microplate kit is used to determine both the activity and quantity of cytochrome c oxidase in a human sample with greater speed and simplicity. The ratio of the two is a measure of specific activity. The COX enzyme is immunocaptured within the wells of the microplate and the activity is determined by following the oxidation of reduced Cytochrome c colorimetrically by the absorbance change at 550 nm. Subsequently, in these same wells the quantity of the enzyme can then be determined by adding a COX specific detector antibody and the appropriate alkaline phophatase conjugated antibody; this phosphatase changes a substrate from colorless to yellow at 405 nm. This reaction takes place in a time dependent manner proportional to the amount of protein captured in the wells. Included in this kit for performance of the multiplexing assay are buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
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说明
Range of complex IV / cytochrome c oxidase assay kits
Biochemical assay - ab239711
Immunocapture with biochemical assay (plate-based)*** - ab109911 (rodent) and ab109909 (human)
*** Most popular assay formatImmunocapture with biochemical assay (dipstick) - ab109878 (rodent) and ab109876 (human)
Immunocapture with biochemical assay and ELISA - ab109910 (human) (this kit)
ELISA - ab179880 (human)
Other related productsReview the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
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平台
Microplate reader
性能
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存放说明
Please refer to protocols. -
组件 标识符 96 tests 20X Buffer 1 x 15ml 96-well microplate (12 strips) 1 unit AP Development Reagent 1 x 400µl AP label 2500X Tube B 1 x 12µl Detector antibody Tube A 1 x 1ml Detergent 1 x 1ml Development buffer Tube 3 1 x 10ml Reagent C (Reduced Cytochrome c) 1 x 1ml Wash Buffer Tube 2 1 x 2ml -
研究领域
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别名
- cco
- COX
- Cytochrome c oxidase
- Cytochrome oxidase
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数据库链接
- Entrez Gene: 4512 Human
- Entrez Gene: 4513 Human
- Entrez Gene: 4514 Human
- Entrez Gene: 1345 Human
- Entrez Gene: 1351 Human
- Entrez Gene: 1329 Human
- Entrez Gene: 1349 Human
- Entrez Gene: 1337 Human
see all
相关产品
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Positive Controls
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Related Products
图片
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab109910 被引用在 11 文献中.
- Perez JM et al. Proteomic Analysis Reveals Low-Dose PARP Inhibitor-Induced Differential Protein Expression in BRCA1-Mutated High-Grade Serous Ovarian Cancer Cells. J Am Soc Mass Spectrom 33:242-250 (2022). PubMed: 34958553
- Yao PJ et al. Mitochondrial Electron Transport Chain Protein Abnormalities Detected in Plasma Extracellular Vesicles in Alzheimer's Disease. Biomedicines 9:N/A (2021). PubMed: 34829816
- Carling PJ et al. Deep phenotyping of peripheral tissue facilitates mechanistic disease stratification in sporadic Parkinson's disease. Prog Neurobiol 187:101772 (2020). PubMed: 32058042
- Richter F et al. ROMO1 is a constituent of the human presequence translocase required for YME1L protease import. J Cell Biol 218:598-614 (2019). PubMed: 30598479
- Aich A et al. COX16 promotes COX2 metallation and assembly during respiratory complex IV biogenesis. Elife 7:N/A (2018). PubMed: 29381136