Complex III免疫捕获试剂盒(ab109800)
Key features and details
- Assay type: Quantitative
概述
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产品名称
Complex III免疫捕获试剂盒
参阅全部 Complex III 试剂盒 -
检测类型
Quantitative -
种属反应性
与反应: Mouse, Rat, Cow, Human -
产品概述
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex III from heart mitochondria.
ab109800 Complex III Immunocapture Kit allows isolation of the ubiquinol-cytochrome c oxidoreductase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for ab109800 include research on genetic mitochondrial disease, where complex III may have an additional role in the stabilization of complex I.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
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说明
Related products
Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
性能
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组件 250 µg monoclonal Complex III antibody irreversibly cross-linked to protein G-agarose beads 1 x 250µg -
研究领域
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相关性
Complex II (succinate-ubiquinone oxidoreductase), one of the mitochondrial respiratory chain complexes, transfers electrons from succinate generated during the citric acid cycle to Complex III (ubiquinolcytochrome c oxidoreductase), via a mobile electron shuttle, ubiquinone. Complex III transfers electrons to Complex IV (cytochrome c oxidase) via another mobile electron shuttle, cytochrome c. -
别名
- coenzyme Q : cytochrome c — oxidoreductase
- cytochrome bc1 complex
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数据库链接
- Entrez Gene: 7384 Human
- Entrez Gene: 7385 Human
- Entrez Gene: 1537 Human
- Entrez Gene: 7386 Human
- Entrez Gene: 4519 Human
- Entrez Gene: 7381 Human
- Entrez Gene: 7388 Human
- Entrez Gene: 27089 Human
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相关产品
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Related Products
图片
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Complex III immunoprecipitation using antibody ab109862/MS301 crosslinked to protein G-agarose beads as product ab109800.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab109800 被引用在 5 文献中.
- Mei L et al. Rieske iron-sulfur protein induces FKBP12.6/RyR2 complex remodeling and subsequent pulmonary hypertension through NF-?B/cyclin D1 pathway. Nat Commun 11:3527 (2020). PubMed: 32669538
- Desmurs M et al. C11orf83, a mitochondrial cardiolipin-binding protein involved in bc1 complex assembly and supercomplex stabilization. Mol Cell Biol 35:1139-56 (2015). PubMed: 25605331
- Korde AS et al. Primary role of mitochondrial Rieske iron-sulfur protein in hypoxic ROS production in pulmonary artery myocytes. Free Radic Biol Med 50:945-52 (2011). PubMed: 21238580
- Munusamy S et al. Alteration of renal respiratory Complex-III during experimental type-1 diabetes. BMC Endocr Disord 9:2 (2009). PubMed: 19166612
- Schilling B et al. Proteomic analysis of succinate dehydrogenase and ubiquinol-cytochrome c reductase (Complex II and III) isolated by immunoprecipitation from bovine and mouse heart mitochondria. Biochim Biophys Acta 1762:213-22 (2006). PubMed: 16120479