Complex I Rodent Protein Quantity试纸检测试剂盒(ab109875)


  • 产品名称Complex I Rodent Protein Quantity试纸检测试剂盒
    参阅全部 Complex I 试剂盒
  • 样品类型
    Cell culture extracts, Tissue
  • 检测类型Sandwich (quantitative)
  • 种属反应性
    与反应: Mouse, Rat
  • 产品概述

    Contains 30 or 90 dipsticks and necessary components to quantify the levels of the fully assembled Complex I enzyme complex from mouse and rat samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.

    Based on the immunologic sandwich assay, the kit utilizes two monoclonal antibodies (mAbs) specific to different antigens present on the Complex I enzyme complex. One antibody is immobilized on the nitrocellulose membrane in a thin line perpendicular to the length of the dipstick, while the other is gold-conjugated and combined with the sample mix. The sample contents containing the gold-conjugated mAb wick past the mAb immobilized on the dipstick. When assembled Complex I is present in the sample, a red line appears at the site of the anti-Complex I antibody line. The signal intensity is directly related to the amount of Complex I in the sample. The signal intensity is best measured by a dipstick reader or may be analyzed by another imaging system. To identify defects in Complex I that do not affect enzyme assembly combine this assay with our Complex I Enzyme Activity Dipstick Assay Kit (ab109720) to determine the relative specific activity of Complex I.

  • 说明

    All components are stable in their provided containers at room temperature out of direct sunlight.

    After diluting the 10X Blocking Buffer to 2X, store at 4°C.

    For long-term storage, all buffers can be stored at 4°C.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Reagents


  • 别名
    • NADH dehydrogenase


Our Abpromise guarantee covers the use of ab109875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Complex I Rodent Protein Quantity Dipstick Assay Kit 图像

  • An example using MS133 to quantify Complex I levels using various concentration of mouse brain tissue. Shown is a 1:2 dilution series using a positive control sample. Approximately 6 to 8 dipsticks are suitable for covering the entire working range and the blank for background levels. In this example the dilution series starts with 20 µg of mouse brain tissue extract. A one-site hyperbola line was generated for best-fit analysis using GraphPad.
  • An example using MS133 to quantify Complex I levels using various concentration of mouse brain tissue. Based on the above results, 13 µg of tissue extract was loaded to determine intra-assay reproducibility. (Note: for a statistical analysis it is preferred to use two dipsticks for each sample; intra-assay CV's are typically <=10%). The average of the signal intensities was determined, and then the amount (µg) of Complex I was interpolated off of the standard curve.
  • Dipstick assays use the well-established lateral flow concept, whereby capture antibodies are striped onto nitrocellulose membrane and a Whatman paper wicking pad draws the sample through the antibody bands. Detector antibodies, conjugated to gold, are dried in the wells of a 96-well plate. Sample is added to the well, the dipstick inserted, and within minutes the line for each target is revealed as the protein-detector antibody-gold complex binds with the capture antibodies. Multiplexing dipstick assays have multiple target protein lines. A positive control goat anti-mouse antibody line is included on all assays to ensure that adequate wicking of the sample occurred.


Complex I Rodent Protein Quantity Dipstick Assay Kit (ab109875)参考文献

ab109875 has not yet been referenced specifically in any publications.

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These kits will be OK to use with snap frozen tissues.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Thank you for contacting us.

The kits are guaranteed for 6 months if stored as recommended on the datasheet.

Please note there are components in the kits that are supposed to stored at different temperatures e.g. -20 or -80C, so we...

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