概述

  • 产品名称Complex I免疫捕获试剂盒
    参阅全部 Complex I 试剂盒
  • 检测类型Quantitative
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 产品概述

    250 µg, 500 µg or 750 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~60 µg, ~120 µg or ~180 µg respectively of Complex I from heart mitochondria.


    ab109711 allows for isolation of Complex I from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains NADH-ferricyanide, NADH-CoQ1 and NADH hexaaminoruthenium reductase activities after isolation, and with added lipids it also shows significant rotenone sensitivity. Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Uses for ab109711 include but are not limited to examining alterations of Complex I subunits in inherited mitochondrial diseases, Parkinson's disease, Alzheimer's disease, ALS, schizophrenia, and aging.


    Note: The immunocapture protocol for this kit requires Abcam detergent (lauryl maltoside, ab109857).


    Product supplied as a 6.25% slurry. For the 250 µg, this consists of 250 µg antibody conjugated to 25 µL beads in a volume of 400 µL.

  • 经测试应用适用于: IPmore details

性能

  • 别名
    • NADH dehydrogenase

应用

Our Abpromise guarantee covers the use of ab109711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution.

Complex I Immunocapture Kit 图像

  • Complex I immunoprecipitation using antibody ab109798/MS101c crosslinked to protein G-agarose beads as product ab109711

实验方案

Complex I Immunocapture Kit (ab109711)参考文献

This product has been referenced in:
  • Jian B  et al. Resveratrol restores sirtuin 1 (SIRT1) activity and pyruvate dehydrogenase kinase 1 (PDK1) expression after hemorrhagic injury in a rat model. Mol Med 20:10-6 (2014). Functional Studies ; Rat . Read more (PubMed: 24395567) »
  • Guarani V  et al. TIMMDC1/C3orf1 functions as a membrane-embedded mitochondrial complex I assembly factor through association with the MCIA complex. Mol Cell Biol 34:847-61 (2014). Read more (PubMed: 24344204) »

See all 15 Publications for this product

Product Wall

Hello! Below is some more information about kit sizes. Please let me know if I can be of further assistance.

The kits size is based on antibody amount not the beads amount. 250ug refers to 25ul beads, 500ug to 50ul beads and 750ug to 75ul bead...

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The lab let me know that both ab109711 and ab109798 are IP antibodies - unfortunately they do not work in WB. One can use it to pull down the whole complex I but needs to use other complex I subunit antibodies to blot the corresponding targets. Read More

To prepare 10% lauryl maltoside as the detergent for ab109711, pure water (deionized, nanopure etc) can be used. The lauryl maltoside should go into solution fairly easily. Gently turn in rotator, try not to shake it too much to prevent frothing.
...

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In general, for well-prepped mitochondria, the ratio is 1-2 mg of mitochondria per 10 uL of beads. The optimal conditions will depend on the quality and source of the mitochondria so it’s very difficult to be specific, however. A good q...

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Hello! Please find the answers to your questions below.

1-elution buffer composition that you recommend in the kit such as 1% SDS.
Is it a buffer or just a water based solution where SDS is solubilized..



It is a water ba...

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Thank you for contacting Abcam regarding A109711.

I have been in contact with the lab regarding your experiences with this product. However as you have tried a number of the troubleshooting steps already they have requested that I ask you fo...

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Thank you for contacting us.



I have contacted our mitochondria-experts in the lab and received the following reply:

We don’t have a lot of experience with adipose tissue. My suggestion would be therefore to isolate...

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Thank you for your enquiry.

The three different elution methods are recommended to different purposes. Proteins eluted with Glycine remain activity, which is suitable for follow up activity assays. Plus the antibody beads can still be resued...

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Thank you for your enquiry. Please find the answers to your questions below.

1) Thank you for your feedback. We will take this into consideration with the production team.

2) Typically customers have used this kit in combination w...

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The MitoSciences laboratory recommends the following: Use 1 mg of brain tissue lysate, eluting into the minimum volume possible. A glycine buffer elution procedure allows the reuse of the beads. If you wish to analyze the product by Weste...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"