Anti-Complement C3抗体(ab117244)

概述

  • 产品名称Anti-Complement C3抗体
  • 描述
    兔多克隆抗体to Complement C3
  • 经测试应用适用于: ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified Human Complement C3 protein.

性能

应用

Our Abpromise guarantee covers the use of ab117244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.

靶标

  • 功能C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
    Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    Acylation stimulating protein (ASP): adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2.
  • 组织特异性Plasma. The acylation stimulating protein (ASP) is expressed in adipocytes and released into the plasma during both the fasting and postprandial periods.
  • 疾病相关Complement component 3 deficiency
    Age-related macular degeneration 9
    Hemolytic uremic syndrome atypical 5
    Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.
  • 序列相似性Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • 翻译后修饰C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g. C3a is further processed by carboxypeptidases to release the C-terminal arginine residue generating the acylation stimulating protein (ASP). Levels of ASP are increased in adipocytes in the postprandial period and by insulin and dietary chylomicrons.
    Phosphorylation sites are present in the extracellular medium.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ASP antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
    • C3 antibody
    • C3a anaphylatoxin antibody
    • C3adesArg antibody
    • CO3_HUMAN antibody
    • Complement C3 alpha chain antibody
    • Complement C3 antibody
    • Complement C3b alpha' chain antibody
    • Complement C3c alpha' chain fragment 1 antibody
    • Complement C3c alpha'' chain fragment 2 antibody
    • Complement C3d fragment antibody
    • Complement C3dg fragment antibody
    • Complement C3f fragment antibody
    • Complement C3g fragment antibody
    • CPAMD1 antibody
    • omplement C3 beta chain antibody
    see all

Anti-Complement C3 antibody (ab117244)参考文献

ab117244 has not yet been referenced specifically in any publications.

Product Wall

In ELISA (antibody capture assay) the titer for this antibody was determined to be 1:25,000.
The recommended protocol is as follow :


Use 96 well ELISA plates
Apply antigen C3 complement about 100 ng/50 ul per well in PBS. Overnig...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"