Anti-COMP抗体[MA37C94 (HC484D1)] (ab11056)

概述

  • 产品名称
    Anti-COMP抗体[MA37C94 (HC484D1)]
    参阅全部 COMP 一抗
  • 描述
    大鼠单克隆抗体[MA37C94 (HC484D1)] to COMP
  • 特异性
    Ab11056 recognises human COMP.
  • 经测试应用
    适用于: WB, IHC-Fr, IHC-P, ELISA, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human cartilage derived COMP

  • 表位
    The antibody recognises an epitope located in the central portion of the molecule. Unfortunately, we do not have information regarding the exact region.
  • 常规说明

    Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

     

性能

应用

Our Abpromise guarantee covers the use of ab11056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

靶标

  • 功能
    May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7.
  • 组织特异性
    Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. Increased amounts are produced during late stages of osteoarthritis in the area adjacent to the main defect.
  • 疾病相关
    Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1) [MIM:132400]. EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types.
    Defects in COMP are the cause of pseudoachondroplasia (PSACH) [MIM:177170]. PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood.
  • 序列相似性
    Belongs to the thrombospondin family.
    Contains 4 EGF-like domains.
    Contains 1 TSP C-terminal (TSPC) domain.
    Contains 8 TSP type-3 repeats.
  • 发展阶段
    Present during the earliest stages of limb maturation and is later found in regions where the joints develop.
  • 结构域
    The cell attachment motif mediates the attachment to chondrocytes. It mediates the induction of both the IAP family of survival proteins and the antiapoptotic response.
    The TSP C-terminal domain mediates interaction with FN1 and ACAN.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple) antibody
    • Cartilage oligomeric matrix protein antibody
    • Cartilage oligomeric matrix protein precursor antibody
    • COMP antibody
    • COMP_HUMAN antibody
    • EDM 1 antibody
    • EDM1 antibody
    • EPD 1 antibody
    • EPD1 antibody
    • Epiphyseal dysplasia 1 antibody
    • Epiphyseal dysplasia 1 multiple antibody
    • Epiphyseal dysplasia multiple 1 antibody
    • MED antibody
    • MGC13181 antibody
    • MGC149768 antibody
    • PSACH antibody
    • pseudoachondroplasia (epiphyseal dysplasia 1, multiple) antibody
    • Pseudoachondroplasia antibody
    • THBS 5 antibody
    • THBS5 antibody
    • Thrombospondin 5 antibody
    • Thrombospondin-5 antibody
    • Thrombospondin5 antibody
    • TSP5 antibody
    see all

文献

This product has been referenced in:
  • Posey KL  et al. Antioxidant and anti-inflammatory agents mitigate pathology in a mouse model of pseudoachondroplasia. Hum Mol Genet 24:3918-28 (2015). IHC ; Mouse . Read more (PubMed: 25859006) »
  • Posey KL  et al. Chondrocyte-specific pathology during skeletal growth and therapeutics in a murine model of pseudoachondroplasia. J Bone Miner Res 29:1258-68 (2014). IHC ; Mouse . Read more (PubMed: 24194321) »

See all 5 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (trochlea bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
trochlea bone
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 0.24% · Temperature: rt°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 06 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0, microwave
Sample
Human Tissue sections (human cartilage)
Specification
human cartilage
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

提交于 Mar 19 2014

DISCOUNT CODE: 100%0000

Ablaufdatum: 22. Oktober 2012

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I"m sorry this vial of ab11056 is giving Xiangyuan trouble. I am happy to provide a refund if this antibody has been purchased within the last 6 months or so. I will just need a PO number or Abcam order reference n...

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Thank you for your enquiry. The antibody ab11056 is a tissue culture supernatant, therefore we can't give a precise concentration. Supernatants will typically be between 10 and 50 ug/ml specific antibody. IHC staining is described in: Di Cesare ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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