Anti-Collagenase抗体- BSA and Azide free (Biotin) (ab182881)
Key features and details
- Biotin Rabbit polyclonal to Collagenase - BSA and Azide free
- Suitable for: IHC-P, ELISA, ICC, WB, Dot blot
- Conjugation: Biotin
- Isotype: IgG
概述
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产品名称
Anti-Collagenase抗体- BSA and Azide free (Biotin)
参阅全部 Collagenase 一抗 -
描述
生物素兔多克隆抗体to Collagenase - BSA and Azide free -
宿主
Rabbit -
偶联物
Biotin -
经测试应用
适用于: IHC-P, ELISA, ICC, WB, Dot blotmore details -
种属反应性
与反应: Clostridium histolyticum -
免疫原
Full length native protein (purified) corresponding to Collagenase. Purified from Clostridium histolyticum.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:reconstitute by adding 1.0 ml sterile distilled water. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.20
Constituent: 100% PBS -
无载体
是 -
Concentration information loading...
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纯度
IgG fraction -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab182881于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/5000 - 1/100000.
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ELISA |
1/5000 - 1/100000.
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ICC |
1/5000 - 1/100000.
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|
WB |
1/5000 - 1/100000.
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|
Dot blot |
1/5000 - 1/100000.
|
说明 |
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IHC-P
1/5000 - 1/100000. |
ELISA
1/5000 - 1/100000. |
ICC
1/5000 - 1/100000. |
WB
1/5000 - 1/100000. |
Dot blot
1/5000 - 1/100000. |
靶标
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相关性
Function: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. Domain: There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases). The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. PTM: Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. Similarity: Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains. -
细胞定位
Secreted, extracellular space, extracellular matrix -
别名
- CLG antibody
- Fibroblast collagenase antibody
- matrix metallopeptidase 1 (interstitial collagenase) antibody
see all
实验方案
数据表及文件
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Datasheet download
文献 (0)
ab182881 尚未被引用在任何文献中。