Anti-Collagen XVII抗体[NC16A-3] (ab79878)

Abreviews (2)Q&A (21)Specific References (1)

概述

  • 产品名称
    Anti-Collagen XVII抗体[NC16A-3]
    参阅全部 Collagen XVII 一抗
  • 描述
    小鼠单克隆抗体[NC16A-3] to Collagen XVII
  • 宿主
    Mouse
  • 经测试应用
    适用于: IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified recombinant NC16a-domain of human collagen XVII

  • 阳性对照
    • Human normal skin tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    NC16A-3
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab79878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/40. For 60 minutes at room temperature for Biotin based detection systems. May require polymer detection. For optimal staining, the sections should be pretreated with an antigen unmasking solution.
ELISA Use at an assay dependent concentration.

靶标

  • 相关性
    Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in the gene coding for collagen XVII are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD 1, is generated by proteolytic processing of the full length form. Two transcript variants, one resulting from alternative splicing in the 3' UTR, have been identified for this gene.
  • 细胞定位
    Cell junction, hemidesmosome. Membrane; Single-pass type II membrane protein. Note=Localized along the plasma membrane of the hemidesmosome. 120 kDa linear IgA disease antigen and 97 kDa linear IgA disease antigen: Secreted, extracellular space, extracellular matrix, basement membrane.
  • 数据库链接
    • 别名
      • 180 kDa bullous pemphigoid antigen 2 antibody
      • Alpha 1 type XVII collagen antibody
      • BA16H23.2 antibody
      • BP 180 antibody
      • BP180 antibody
      • BPA 2 antibody
      • BPAG 2 antibody
      • BPAG2 antibody
      • Bullous pemphigoid antigen 2 antibody
      • COL17A1 antibody
      • Collagen 17 antibody
      • Collagen alpha 1 XVII chain antibody
      • Collagen alpha 1(XVII) chain antibody
      • Collagen alpha1 XVII chain antibody
      • Collagen type XVII alpha 1 antibody
      • Collagen XVII alpha 1 polypeptide antibody
      • CollagenXVII antibody
      • Epidermolysis bullosa junctional localisata variant antibody
      • FLJ60881 antibody
      • KIAA0204 antibody
      • LAD 1 antibody
      • LAD1 antibody
      see all

    文献

    This product has been referenced in:

    See 1 Publication for this product

    发表研究结果有使用 ab79878？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    23 reviews or Q&A

    10
    Votes: Highest First

    ELISA abreview for Anti-Collagen XVII antibody [NC16A-3]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    ELISA
    Sample
    Human Purified protein (purified protein)
    Specification
    purified protein
    Blocking step
    roch blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Type
    Direct
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    Username

    Abcam user community

    Verified customer

    提交于 Mar 01 2013

    I would be happy to test another vial.
    Kind regards,

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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    Thanksfor the offer of 10% off our next NC16-A3 order.
    Unfortunatley that seems a bit light given the costs involved and the fact that you are offering essentially similar lots (from the same mother stock) as we ordered in May 2012 (GR85966). I was...

    Read More

    I have now received a reply from lab; they unfortunately have same lot in stock. They have agreed to retest the lot, I will share the results as soon as I receive from them.

    This antibody is stored at 4C and we guarantee its viability for 1 ye...

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    Unfortunatley that seems a bit light given the costs involved and the fact that you are offering essentially similar lots (from the same mother stock) as we ordered in May 2012 (GR85966). I was wondering if this really is that the best you can do?
    ...

    Read More

    I am having discussion with lab regarding the new lot. I will get back to you soon.

    Many thanks for having patience!

    Thanks for you prompt reply.
    We have already optimized this and have had no other probelms with any of the 8 other mouse antibodies we also routinely use.

    Thats just leaves the possibility of variation between the different batches.

    Read More

    Thank you very much for your email.

    You can order the antibody with usual ordering process from our website. We have two lots GR85966-1, GR85966-2 available in stock; these are however from same mother stock. Is this lot number different than...

    Read More

    Apologies for the delay with this reply. We were being Clinical Pathology accredited which we have now completed.

    We are using cryostat fresh-cut(unfixed) human skin tissue for all our tests.

    We have tested this NC16A-3 antibody on p...

    Read More

    Thank you very much for your email.

    I am sorry we could not find a reason, why the particularantibodyvial isactive than expected. All the lots we produced passed the strict quality control criteria however we can not deny small variations due ...

    Read More


    Thanks for your answer. Epidermolysis Bullosa itself is the difficult issue so that we accept that all.

    I'm happy to help! Best of luck in your research. Feel free to contact me if you need anything further.

    As checking ab79878 datasheet, I think it should work well so that I would like to check again with this new antibody (in case the old one was wrong due to transport or other reasons that we don't know). On the other side, I have not found any other op...

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    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

    To check the status of the order please contact our Customer Service team...

    Read More




    We did not receive anyinformation aboutthe goods



    would like to change to collagen17, ab79878.



    I hope to receive your answer soonest

    Thanks for your email.

    I am sorry for any confusion here. I believe we were just waiting to find out which antibody you would like as the free replacement for the faulty vial of ab79878 you received in February of this year. If you wish I am ...

    Read More

    Ich habe gerade gesehen, dass der Antikörper ab28440 gegen ein Fusionsprotein gerichtet ist. Ich würde dann lieber ab79878 bestellen. Das sollte doch für den Gutscheincode keinen Unterschied machen, oder?
    Viele Grüße

    Vielen Dank für Ihre Antwort.

    Der Code ist an einen spezifischen Antikörper gebunden und kann nicht für ab79878 verwendet werden. ich werde Ihnen aber gerne einen neuen Code für ab79878 ausstellen.

    Ich möcht...

    Read More

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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