This antibody gave a positive signal in the following Human tissue lysates: Heart; Kidney; Spleen; Liver; Lung.
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Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 73 kDa).
Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in artherogenesis. Vastatin, the C-terminal fragment comprising the NC1 domain, inhibits aortic endothelial cell proliferation and causes cell apoptosis.
Expressed primarily in the subendothelium of large blood vessels. Also expressed in arterioles and venules in muscle, heart, kidney, spleen, umbilical cord, liver and lung and is also found in connective tissue layers around hair follicles, around nerve bundles in muscle, in the dura of the optic nerve, in cornea and sclera, and in the perichondrium of cartilaginous tissues. In the kidney, expressed in mesangial cells, glomerular endothelial cells, and tubular epithelial cells. Also expressed in mast cells, and in astrocytes during the repair process. Expressed in Descemet's membrane. Specifically expressed in peritoneal fibroblasts and mesothelial cells.
Contains 1 C1q domain.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proteolytically cleaved by neutrophil elastase, in vitro. Proteolytic processing produces the C-terminal NC1 domain fragment, vastatin.
Secreted > extracellular space > extracellular matrix > basement membrane.
Western blot - Anti-Collagen VIII alpha 1 antibody (ab100988)
All lanes : Anti-Collagen VIII alpha 1 antibody (ab100988) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431) Lane 2 : Human kidney tissue lysate - total protein (ab30203) Lane 3 : Human spleen tissue lysate - total protein (ab29699) Lane 4 : Human liver tissue lysate - total protein (ab29889) Lane 5 : Lung (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
The band observed at 62 kDa could potentially be a cleaved form of Collagen VIII alpha 1 due to the presence of a 27 amino acid signal peptide.
The expression profile observed is consistent with what has been described in the literature (PMID:8921010, PMID:8781485, PMID:10428768, PubMed: 19109829).