Anti-Collagen VII抗体(ab93350)


  • 产品名称
    Anti-Collagen VII抗体
    参阅全部 Collagen VII 一抗
  • 描述
    兔多克隆抗体to Collagen VII
  • 宿主
  • 经测试应用
    适用于: WB, IP, ELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Human, Common marmoset
  • 免疫原

    Full length native protein (purified) corresponding to Human Collagen VII.

  • 阳性对照
    • Human skin tissue.



Our Abpromise guarantee covers the use of ab93350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明
    ELISA: Use at an assay dependent dilution.
    EIA: Use at an assay dependent dilution.
    Flow Cyt: Use at an assay dependent dilution.
    ICC/IF: Use at a concentration of 2.5 - 10 µg/ml.
    IP: Use at a concentration of 2.5 - 10 µg/ml.
    WB: Use at a concentration of 0.2 - 0.8 µg/ml. Predicted molecular weight: 295 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
    • 疾病相关
      Epidermolysis bullosa acquisita (EBA) is an autoimmune acquired blistering skin disease resulting from autoantibodies to type VII collagen.
      Epidermolysis bullosa dystrophica, autosomal dominant
      Epidermolysis bullosa dystrophica, autosomal recessive
      Epidermolysis bullosa dystrophica, Pasini type
      Epidermolysis bullosa dystrophica, Hallopeau-Siemens type
      Transient bullous dermolysis of the newborn
      Epidermolysis bullosa dystrophica, pretibial type
      Epidermolysis bullosa dystrophica, Bart type
      Epidermolysis bullosa pruriginosa
      Nail disorder, non-syndromic congenital, 8
      Epidermolysis bullosa dystrophica, with subcorneal cleavage
    • 序列相似性
      Contains 1 BPTI/Kunitz inhibitor domain.
      Contains 9 fibronectin type-III domains.
      Contains 2 VWFA domains.
    • 翻译后修饰
      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    • 细胞定位
      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Alpha 1 type VII collagen antibody
      • CO7A1_HUMAN antibody
      • Col7a1 antibody
      • Collagen 7 antibody
      • Collagen alpha 1(VII) chain antibody
      • Collagen alpha-1(VII) chain antibody
      • Collagen type VII alpha 1 antibody
      • Collagen VII alpha 1 polypeptide antibody
      • CollagenVII antibody
      • EBD 1 antibody
      • EBD1 antibody
      • EBDCT antibody
      • EBR 1 antibody
      • EBR1 antibody
      • LC collagen antibody
      • Long chain collagen antibody
      • Long-chain collagen antibody
      see all


    • Immunohistochemical staining of Collagen VII in human skin tissue sections with ab93350. Samples were fixed with formaldehyde and enzymatic antigen retrieval was performed with Proteinase K. Tissue was blocked with 1% BSA for 10 minutes at 21°C before ab93350 was added (1/250) for 2 hours at 21°C. A biotin conjugated goat anti-rabbit secondary was used at 1/250.

      See Abreview

    • Immunohistochemical staining of human skin tissue (frozen sections) labeling Collagen VII with ab93350. Samples were fixed using acetone and 3% BSA was used as blocking agent for 1 hour at room temperature. Samples were incubated with ab93350 for 1 hour at 1/200 dilution. An Alexa Fluor-488 rabbit polyclonal was used as a secondary antibody.

      See Abreview

    • Immunofluorescence staining of Collagen VII in normal human keratinocytes. Second antibody for staining of cells: goat-anti-rabbit IgG Alexa 488


    This product has been referenced in:
    • Gouveia RM  et al. Controlling the 3D architecture of Self-Lifting Auto-generated Tissue Equivalents (SLATEs) for optimized corneal graft composition and stability. Biomaterials 121:205-219 (2017). IF ; Human . Read more (PubMed: 28092777) »
    • Breitenbach JS  et al. Transcriptome and ultrastructural changes in dystrophic Epidermolysis bullosa resemble skin aging. Aging (Albany NY) 7:389-411 (2015). IF . Read more (PubMed: 26143532) »

    See all 2 Publications for this product


    Thank you for your inquiry.

    I heard back from the lab that our anti-Collagen VII antibody is specific only for helical domains (collagen VII fragments). The non-collagen fragments obtained after pepsin digestion are broken and cannot be detec...

    Read More

    Thank you for contacting us.
    Both antibodies were run under reducing and denaturing conditions.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use o...

    Read More

    Thank you for your enquiry.

    I can confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products. However, in ma...

    Read More

    Thank you for contacting us. The BLAST results show 84% homology between the full-length human (immunogen) and mouse and is likely to work. If you are interested in this product we do have a testing discount program which acts as a buy one get one free...

    Read More