Anti-Collagen VI抗体(Biotin) (ab6583)

概述

  • 产品名称Anti-Collagen VI抗体(Biotin)
    参阅全部 Collagen VI 一抗
  • 描述
    兔多克隆抗体to Collagen VI (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: ELISA, IHC-Fr, IP, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Cow, Human
    预测可用于: all Mammals
  • 免疫原

    Collagen Type VI from adult human and bovine tissues

  • 常规说明At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 8.00
    Preservative: 0.01% Sodium azide
    Constituents: 0.44% Sodium chloride, 1% BSA, 4.77% Sodium borate, 0.146% EDTA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/4000 - 1/8000.
IHC-Fr 1/50 - 1/200.
IP Use at an assay dependent concentration.
WB 1/5000 - 1/10000. This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Collagen VI acts as a cell-binding protein.
  • 疾病相关Defects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
    Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy.
  • 序列相似性Belongs to the type VI collagen family.
    Contains 3 VWFA domains.
  • 翻译后修饰Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha 1 (VI) chain (61 AA) antibody
    • CO6A1_HUMAN antibody
    • COL6A1 antibody
    • COL6A2 antibody
    • COL6A3 antibody
    • Collagen alpha 2(VI) chain antibody
    • Collagen alpha 3(VI) chain antibody
    • Collagen alpha-1(VI) chain antibody
    • Collagen type VI alpha 1 antibody
    • Collagen type VI alpha 2 antibody
    • Collagen type VI alpha 3 antibody
    • Collagen VI alpha 1 polypeptide antibody
    • Collagen VI alpha 2 polypeptide antibody
    • Collagen VI alpha 3 polypeptide antibody
    • CollagenVI antibody
    • Human mRNA for collagen VI alpha 2 C terminal globular domain antibody
    • OPLL antibody
    • PP3610 antibody
    see all

Anti-Collagen VI antibody (Biotin) 图像

  • ab6583 staining Collagen VI (red) in Bovine bone marrow stromal cells (BMSCs) and articular chondrocytes (ACs) by ICC/IF (Immunocytochemistry/immunofluorescence). Sections were fixed with formalin and blocked with 5% BSA for 30 minutes. Samples were incubated with primary antibody (10 μg/ml). An Alexa Fluor®-conjugated anti-rabbit IgG was used as the secondary antibody. Decorin - green, DNA - blue.

  • ab6583 staining Collagen VI in rabbit decalcified collagen defective femur by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed using Proteinase K. Samples were then blocked using 100% avidin/biotin for 10 minutes and then incubated with ab6583 at a 1/50 dilution for 1 hour. Streptavidin-HRP was used for detection of biotinylated primary.

    See Abreview

Anti-Collagen VI antibody (Biotin) (ab6583)参考文献

This product has been referenced in:
  • Ponsioen TL  et al. Human retinal Müller cells synthesize collagens of the vitreous and vitreoretinal interface in vitro. Mol Vis 14:652-60 (2008). ICC/IF ; Human . Read more (PubMed: 18385800) »
  • Ponsioen TL  et al. Collagen distribution in the human vitreoretinal interface. Invest Ophthalmol Vis Sci 49:4089-95 (2008). IHC-P ; Human . Read more (PubMed: 18450587) »

See all 3 Publications for this product

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Thank you for contacting us with your question.

Ab6583 has not been tested for reactivity with mouse samples at this time, andthough we predict it to react based on sequence homology, we are not able to guarantee reactivity. What kind ofexper...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rabbit Tissue sections (Decalcified Collagen Defect Femur)
Specification Decalcified Collagen Defect Femur
Fixative Formaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization No
Blocking step Avidin/Biotin as blocking agent for 10 minute(s) · Concentration: 100%
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提交于 Dec 28 2011

DISCOUNT CODE: ******* Expiration date: ******* I am very pleased to hear you would like to accept our offer and test ab6583 in IHC-P. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Ab...

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For ab6583, the band size is 560 kDa under non-reducing. Band size under reducing conditions is unknown. The antibody won't be specfic to reduced collagen VI. Ab6311 specifically recognizes native, undenatured, human collagen type IV and recognise...

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Thank you very much for your enquiry. All the information that we have regarding these antibodies is located on the online datasheets, please refer to them for more information. Here are the expected band sizes for these antibodies: ab7045 - 21.6 kDa ...

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The concentration was 1.5mg/mL. We sell 100ug product instead of 100uL.

We suggest non-denaturing, non-dissociating gel for anti-Collagen antibodies. Please reference the book: Gel Electrophoresis of Proteins, A Practical Approach, edited by B.D. Hames and D. Rickwood.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"