概述

  • 产品名称
    Anti-Collagen VI抗体
    参阅全部 Collagen VI 一抗
  • 描述
    兔多克隆抗体to Collagen VI
  • 特异性
    Negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
  • 经测试应用
    适用于: ICC/IF, ELISA, IHC-Fr, IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Human, Pig
    预测可用于: Mammal
  • 免疫原

    Full length native protein (purified) corresponding to Human Collagen VI aa 1-1028. Collagen Type VI from human and bovine placenta.
    Database link: P12109

  • 阳性对照
    • HepG2. Pig retinal pigment epithelium. Human placenta.
  • 常规说明

    At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 8.00
    Preservative: 0.01% Sodium azide
    Constituents: 0.44% Sodium chloride, 4.8% Sodium borate, 0.15% EDTA
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/250 - 1/500.
ELISA 1/4000 - 1/8000.
IHC-Fr 1/50 - 1/200.
IP Use at an assay dependent concentration.
WB 1/5000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 108 kDa). This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.
IHC-P Use at an assay dependent concentration. PubMed: 18065394

靶标

  • 功能
    Collagen VI acts as a cell-binding protein.
  • 疾病相关
    Defects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
    Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy.
  • 序列相似性
    Belongs to the type VI collagen family.
    Contains 3 VWFA domains.
  • 翻译后修饰
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha 1 (VI) chain (61 AA) antibody
    • CO6A1_HUMAN antibody
    • COL6A1 antibody
    • COL6A2 antibody
    • COL6A3 antibody
    • Collagen alpha 2(VI) chain antibody
    • Collagen alpha 3(VI) chain antibody
    • Collagen alpha-1(VI) chain antibody
    • Collagen type VI alpha 1 antibody
    • Collagen type VI alpha 2 antibody
    • Collagen type VI alpha 3 antibody
    • Collagen VI alpha 1 polypeptide antibody
    • Collagen VI alpha 2 polypeptide antibody
    • Collagen VI alpha 3 polypeptide antibody
    • CollagenVI antibody
    • Human mRNA for collagen VI alpha 2 C terminal globular domain antibody
    • OPLL antibody
    • PP3610 antibody
    see all

Anti-Collagen VI antibody 图像

  • Anti-Collagen VI antibody (ab6588) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 108 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of 10% buffered formalin-fixed swine lung tissue sections, labelling Collagen VI with ab6588 at a dilution of 1/400 incubated for 12 hours at 4°C. Antigen retrival was with 10mM sodium citrate buffer pH 6.0 (heat mediated). Blocking was with 5% serum incubated for 1 hour at 21°C. The secondary was a Donkey anti-Rabbit polyclonal Alexa Fluor® 647 conjugtate undiluted.

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  • ab6588 staining (green) Collagen VI in a primary cell culture from Mouse retinal pigment epithelium by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody 1/500 (1% goat serum, 0.1% TX100, 1XPBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat polyclonal to rabbit IgG (ab150077), dilution 1/500, was used as secondary antibody. Nuclei were counterstained with DAPI (blue).

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  • ab6588 staining Collagen VI in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and an antigen retrieval step was performed using citrate buffer. Samples were then blocked with 10% serum for 30 minutes at 20°C and then incubated with ab6588 at a 1/50 dilution for 1 hour at 37°C. The secondary used was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal (ab150078), used at a 1/500 dilution.

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  • ab6588 staining Collagen VI in a primary cell culture from Pig retinal pigment epithelium by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody 1/250 (1% goat serum, 0.1% TX100, 1XPBS)  for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat polyclonal to rabbit IgG, dilution 1/500, was used as secondary antibody. Nuclei were counterstained with DAPI (blue).

    See Abreview

  • ab6588 at 1/400 dilution staining Collagen VI in Human placenta (Top) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.

  • Immunohistochemical analysis of Formaldehyde fixed human colon tissue sections labelling Collagen VI with ab6588 at a dilution of 1/200. The secondary antibody used was HRP conjugated rabbit polyclonal secondary. Antigen retrieval was heat mediated. 

    See Abreview

Anti-Collagen VI antibody (ab6588)参考文献

This product has been referenced in:
  • Stribos EGD  et al. Non-invasive quantification of collagen turnover in renal transplant recipients. PLoS One 12:e0175898 (2017). IHC-P ; Human . Read more (PubMed: 28430784) »
  • Blok EJ  et al. Combined evaluation of the FAS cell surface death receptor and CD8+ tumor infiltrating lymphocytes as a prognostic biomarker in breast cancer. Oncotarget 8:15610-15620 (2017). IHC-P ; Human . Read more (PubMed: 28121628) »

See all 42 Publications for this product

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Dermal Fibroblast)
Permeabilization
No
Specification
Dermal Fibroblast
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Adrian Djalali Cuevas

Verified customer

提交于 Sep 26 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue (Cardiac tissue)
Permeabilization
No
Specification
Cardiac tissue
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 31 2017

Application
IHC - Wholemount
Sample
Mouse Tissue (intestine organoids)
Specification
intestine organoids
Username

Abcam user community

Verified customer

提交于 Mar 29 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (adipocyte)
Permeabilization
Yes - 0.05% TX-100
Specification
adipocyte
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 24 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin
Username

Abcam user community

Verified customer

提交于 Sep 07 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin
Username

Abcam user community

Verified customer

提交于 Sep 01 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (artery)
Permeabilization
No
Specification
artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 29 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
bone
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin
Username

Abcam user community

Verified customer

提交于 Jan 28 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium citrate buffer pH 6
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% buffered formalin
Username

Abcam user community

Verified customer

提交于 Sep 30 2015

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