Anti-Collagen V抗体(Biotin) (ab6582)

概述

  • 产品名称Anti-Collagen V抗体(Biotin)
    参阅全部 Collagen V 一抗
  • 描述
    兔多克隆抗体to Collagen V (Biotin)
  • 偶联物Biotin
  • 特异性Negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
  • 经测试应用适用于: IHC-Fr, IP, WB, Indirect ELISAmore details
  • 种属反应性
    与反应: Cow, Human
    预测可用于: all Mammals
  • 免疫原

    Collagen Type V from adult human and bovine tissues

  • 常规说明At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 8
    Preservative: 0.01% Sodium azide
    Constituents: 0.44% Sodium chloride, 1% BSA, 4.77% Sodium borate, 0.146% EDTA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr
IP
WB
Indirect ELISA
  • 应用说明Immunohistochemistry on frozen tissue sections (1:50 to 1:200)
    Indirect trapping ELISA (1:4,000 to 1:8,000) for quantitation of antigen in serum using a standard curve.
    Immunoprecipitation and western blotting (1:5,000 to 1:10,000) for highly sensitive qualitative analysis.

    Optimal titers for other applications should be determined by the researcher.
    Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used
  • 靶标

    • 功能Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
    • 疾病相关Ehlers-Danlos syndrome 1
      Ehlers-Danlos syndrome 2
    • 序列相似性Belongs to the fibrillar collagen family.
      Contains 1 fibrillar collagen NC1 domain.
      Contains 1 laminin G-like domain.
    • 结构域The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
    • 翻译后修饰Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      Sulfated on 40% of tyrosines.
    • 细胞定位Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AB collagen antibody
      • Alpha 1 type V collagen antibody
      • Alpha 2 type V collagen antibody
      • CO5A1_HUMAN antibody
      • COL5A1 antibody
      • Col5A2 antibody
      • COL5A2 protein antibody
      • Col5A3 antibody
      • Collagen alpha 1(V) chain antibody
      • Collagen alpha 2 (V) chain precursor antibody
      • Collagen alpha 2(V) chain antibody
      • Collagen alpha 3(V) chain antibody
      • Collagen alpha-1(V) chain antibody
      • Collagen fetal membrane A polypeptide antibody
      • Collagen type V alpha 1 antibody
      • Collagen type V alpha 2 antibody
      • Collagen type V alpha 3 antibody
      • Collagen V alpha 2 polypeptide antibody
      • CollagenV antibody
      • MGC105115 antibody
      • OTTHUMP00000064637 antibody
      • Pro alpha 1 type V collagen antibody
      • Pro alpha 3(V) collagen antibody
      • Procollagen alpha 2(V) antibody
      • Type V preprocollagen alpha 2 chain antibody
      see all

    Anti-Collagen V antibody (Biotin) (ab6582)参考文献

    ab6582 has not yet been referenced specifically in any publications.

    Product Wall

    The immunogen of the antibody is from Human Collagen V. It cross-reacts with Bovine Collagen V too.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"