概述

  • 产品名称Anti-Collagen V抗体
    参阅全部 Collagen V 一抗
  • 描述
    兔多克隆抗体to Collagen V
  • 特异性Negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
  • 经测试应用适用于: ICC, Sandwich ELISA, IHC-Fr, IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Cow, Human
  • 免疫原

    Full length native protein (purified) corresponding to Collagen V aa 1-1745.

  • 阳性对照
    • IHC :Dog skeletal muscle tissue, Human pancreas tissue.
  • 常规说明

    Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液pH: 8.00
    Preservative: 0.01% Sodium azide
    Constituents: 4.8% Sodium borate, 0.15% EDTA, 0.44% Sodium chloride
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration. PubMed: 18385800
Sandwich ELISA Use a concentration of 10 µg/ml. Can be used with a suitable biotinylated detection antibody such as Rabbit polyclonal to Collagen V (Biotin) (ab6582).
IHC-Fr 1/50 - 1/200.
IP 1/5000 - 1/10000.
WB 1/5000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa). Native conditions are recommended.
IHC-P 1/50 - 1/200.

靶标

  • 功能Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
  • 疾病相关Ehlers-Danlos syndrome 1
    Ehlers-Danlos syndrome 2
  • 序列相似性Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 laminin G-like domain.
  • 结构域The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
  • 翻译后修饰Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Sulfated on 40% of tyrosines.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AB collagen antibody
    • Alpha 1 type V collagen antibody
    • Alpha 2 type V collagen antibody
    • CO5A1_HUMAN antibody
    • COL5A1 antibody
    • Col5A2 antibody
    • COL5A2 protein antibody
    • Col5A3 antibody
    • Collagen alpha 1(V) chain antibody
    • Collagen alpha 2 (V) chain precursor antibody
    • Collagen alpha 2(V) chain antibody
    • Collagen alpha 3(V) chain antibody
    • Collagen alpha-1(V) chain antibody
    • Collagen fetal membrane A polypeptide antibody
    • Collagen type V alpha 1 antibody
    • Collagen type V alpha 2 antibody
    • Collagen type V alpha 3 antibody
    • Collagen V alpha 2 polypeptide antibody
    • CollagenV antibody
    • MGC105115 antibody
    • OTTHUMP00000064637 antibody
    • Pro alpha 1 type V collagen antibody
    • Pro alpha 3(V) collagen antibody
    • Procollagen alpha 2(V) antibody
    • Type V preprocollagen alpha 2 chain antibody
    see all

Anti-Collagen V antibody 图像

  • ab7046 staining Collagen V in Human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate pH 6 prior to blocking with 0.25% casein for 5 minutes at 25°C. The primary antibody was diluted 1/75 in Tris--HCL and incubated with the sample for 30 minutes at 25°C. An HRP polymer-conjugated goat anti-rabbit antibody was used as the secondary antibody.

    See Abreview

  • ab7046 staining Collagen V in Dog skeletal muscle tissue by Immunohistochemistry (Frozen sections). The sections were fixed in Acetone. The primary antibody was diluted 1/100 in 10% Normal Goat Serum (ab7481) in PBS-T and incubated with the sample for 12 hours at 4°C. A Rhodamine-conjugated Goat anti-Rabbit IgG was used as the secondary antibody, diluted 1/200.

    See Abreview

  • Anti-Collagen V antibody (ab7046) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 180 kDa
    Observed band size : 180 kDa
    Additional bands at : 122 kDa,54 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung (left) and placenta (right) sections labelling collagen V with ab7046 at a dilution of 1/200 for 45 minutes at room temperature. An antigen retrival step was performed with 0.01 M sodium citrate buffer pH 6.0 at 100ºC for 20 mins.

Anti-Collagen V antibody (ab7046)参考文献

This product has been referenced in:
  • Baumann S & Hennet T Collagen Accumulation in Osteosarcoma Cells lacking GLT25D1 Collagen Galactosyltransferase. J Biol Chem 291:18514-24 (2016). Read more (PubMed: 27402836) »
  • Camp JG  et al. Human cerebral organoids recapitulate gene expression programs of fetal neocortex development. Proc Natl Acad Sci U S A 112:15672-7 (2015). IHC-Fr . Read more (PubMed: 26644564) »

See all 7 Publications for this product

Product Wall

Thank you for contacting us with your enquiry.

Ab27478 is a suitable isotype control to use with an unconjugated rabbit polyclonal primary antibody, so I would recommend using it as a negative controlfor ab100810 and ab7046 in IHC. As the IgG...

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Thank you for your inquiry.

For a positive control for ab7046, you can use human pancreas tissue slides as ab4611.

http://www.abcam.com/Pancreas-human-normal-tissue-slides-ab4611.html

For the negative control, you can use ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Pancreas)
Specification Pancreas
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization No
Blocking step (agent) for 5 minute(s) · Concentration: .25% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 01 2011

Application Immunohistochemistry (Frozen sections)
Sample Dog Tissue sections (skeletal muscle frozen sections)
Specification skeletal muscle frozen sections
Fixative Acetone
Permeabilization No
Username

Abcam user community

Verified customer

提交于 Sep 20 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (adrenal cortex)
Specification adrenal cortex
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Sep 12 2007

Thank you for your email. I'm afraid that I do not know how neutralizing the pH in your particular assay is going to affect collagen V. To our knowledge this antibody has not been tested in proliferation assays. I'm sorry that I can't be of further ass...

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You want to add glycerol to 50% before freezing. So what your labmate did (vial is 50% antibody, 50% glycerol) is correct.

Thank you for your patience in awaiting a response. Collagen V is over 400kDa.It is important to note that this product will not be supported in reduced conditions. Please use this product in non-reducing conditions. The public-facing data sheet. Thank...

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Sorry, we really don't know if the antibody would detect the type specific collagen fragments.

The immunogen used to generate Rabbit polyclonal to Collagen V was Collagen Type V from adult human and bovine tissues. As the antibody is a polyclonal and was raised agaisnt the whole collagen molecule it will recognise a number of epitopes on the sur...

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