概述

  • 产品名称Anti-Collagen V抗体
    参阅全部 Collagen V 一抗
  • 描述
    兔多克隆抗体to Collagen V
  • 特异性This antibody recognises Collagen Type V and it has negligible cross-reactivity with Type I,II, III, IV, or VI collagens. Non-specific cross reaction with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
  • 经测试应用适用于: WB, IP, IHC-Fr, ELISAmore details
  • 种属反应性
    与反应: Cow, Human
    预测可用于: all Mammals
  • 免疫原

    Collagen Type V from Human and Bovine placenta.

  • 常规说明Antibody concentration is determined by UV absorbance at 280 nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 0.125M Sodium borate, 0.075M Sodium chloride, 0.005M EDTA. pH 8.0
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab34714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
IHC-Fr
ELISA
  • 应用说明ELISA: 1/4000 - 1/8000.
    IHC-Fr: 1/50 - 1/200.
    IP: Use at an assay dependent dilution.
    WB: 1/5000 - 1/10,000. Predicted molecular weight: 180 kDa.

    Denaturing and reducing conditions will greatly diminish reactivity and selectivity of this antibody.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
    • 疾病相关Ehlers-Danlos syndrome 1
      Ehlers-Danlos syndrome 2
    • 序列相似性Belongs to the fibrillar collagen family.
      Contains 1 fibrillar collagen NC1 domain.
      Contains 1 laminin G-like domain.
    • 结构域The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
    • 翻译后修饰Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      Sulfated on 40% of tyrosines.
    • 细胞定位Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AB collagen antibody
      • Alpha 1 type V collagen antibody
      • Alpha 2 type V collagen antibody
      • CO5A1_HUMAN antibody
      • COL5A1 antibody
      • Col5A2 antibody
      • COL5A2 protein antibody
      • Col5A3 antibody
      • Collagen alpha 1(V) chain antibody
      • Collagen alpha 2 (V) chain precursor antibody
      • Collagen alpha 2(V) chain antibody
      • Collagen alpha 3(V) chain antibody
      • Collagen alpha-1(V) chain antibody
      • Collagen fetal membrane A polypeptide antibody
      • Collagen type V alpha 1 antibody
      • Collagen type V alpha 2 antibody
      • Collagen type V alpha 3 antibody
      • Collagen V alpha 2 polypeptide antibody
      • CollagenV antibody
      • MGC105115 antibody
      • OTTHUMP00000064637 antibody
      • Pro alpha 1 type V collagen antibody
      • Pro alpha 3(V) collagen antibody
      • Procollagen alpha 2(V) antibody
      • Type V preprocollagen alpha 2 chain antibody
      see all

    Anti-Collagen V antibody (ab34714)参考文献

    ab34714 has not yet been referenced specifically in any publications.

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