概述

  • 产品名称Anti-Collagen V抗体
    参阅全部 Collagen V 一抗
  • 描述
    兔多克隆抗体to Collagen V
  • 特异性Cross-Reactivity (RIA%) Rat Collagen Type V 100% Rat Collagen Types I, III <0.1% Rat Elastin <0.1%
  • 经测试应用适用于: ELISA, ICC/IF, RIAmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Collagen type V extracted and purified from rat skin.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Buffer: Not applicable.
    Preservative: None
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Ion exchange chromatography (DEAE-Trisacryl)
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab21299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
ICC/IF
RIA
  • 应用说明ELISA: Use at >1:100-1:200 dilution.
    IF: Use at an assay dependent dilution. In indirect IFA with fluorescein anti-rabbit IgG conjugate, use diluted at >1:40 on frozen rat tissue sections.
    (SP)RIA: Use at an assay dependent dilution.
    For immunostaining of extra or intracellular components in light microscopy. For the quantitation of Collagen Type V in biological fluids or in culture supernatants.
    Each laboratory should determine an optimum working titer for use in its particular application.
  • 靶标

    • 功能Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
    • 疾病相关Ehlers-Danlos syndrome 1
      Ehlers-Danlos syndrome 2
    • 序列相似性Belongs to the fibrillar collagen family.
      Contains 1 fibrillar collagen NC1 domain.
      Contains 1 laminin G-like domain.
    • 结构域The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
    • 翻译后修饰Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      Sulfated on 40% of tyrosines.
    • 细胞定位Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AB collagen antibody
      • Alpha 1 type V collagen antibody
      • Alpha 2 type V collagen antibody
      • CO5A1_HUMAN antibody
      • COL5A1 antibody
      • Col5A2 antibody
      • COL5A2 protein antibody
      • Col5A3 antibody
      • Collagen alpha 1(V) chain antibody
      • Collagen alpha 2 (V) chain precursor antibody
      • Collagen alpha 2(V) chain antibody
      • Collagen alpha 3(V) chain antibody
      • Collagen alpha-1(V) chain antibody
      • Collagen fetal membrane A polypeptide antibody
      • Collagen type V alpha 1 antibody
      • Collagen type V alpha 2 antibody
      • Collagen type V alpha 3 antibody
      • Collagen V alpha 2 polypeptide antibody
      • CollagenV antibody
      • MGC105115 antibody
      • OTTHUMP00000064637 antibody
      • Pro alpha 1 type V collagen antibody
      • Pro alpha 3(V) collagen antibody
      • Procollagen alpha 2(V) antibody
      • Type V preprocollagen alpha 2 chain antibody
      see all

    Anti-Collagen V antibody (ab21299)参考文献

    ab21299 has not yet been referenced specifically in any publications.

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