Anti-Collagen V抗体[1.BB.755] (ab62426)

概述

  • 产品名称Anti-Collagen V抗体[1.BB.755]
    参阅全部 Collagen V 一抗
  • 描述
    小鼠单克隆抗体[1.BB.755] to Collagen V
  • 特异性This antibody recognizes native human as well as heat denatured human collagen type V in RIA, ELISA and immunoblot assays. It does not react with other collagen types I, II, III and IV. Human plasma proteins do not interfere with binding to collagen.
  • 经测试应用适用于: ELISA, RIA, IHC-Frmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Collagen Type V (Human)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: Ascites
  • Concentration information loading...
  • 纯化说明Purified by PEG6000 precipitation and Sepharose 6B gel filtration.
  • 克隆单克隆
  • 克隆编号1.BB.755
  • 同种型IgM
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab62426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
RIA
Immunohistochemistry acetone fixed
IHC-Fr
  • 应用说明ELISA: 1/1000. This antibody can also be used for quantitative sandwich ELISA to determine concentration of collagen type V in human fluids.
    IHC-Fr: Use at an assay dependent dilution.
    RIA: Use at an assay dependent dilution.

    Is unsuitable for IHC-P.

    For staining of tissue sections it is advised to use acetone or ethanol fixed cryostat sections, results of staining of paraffin embedded tissues, even after treatment with proteolytic enzymes or microwave, are less satisfactory. The antibody has to be diluted 1:20, if FITC- labeled secondary antibodies are used.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
    • 疾病相关Ehlers-Danlos syndrome 1
      Ehlers-Danlos syndrome 2
    • 序列相似性Belongs to the fibrillar collagen family.
      Contains 1 fibrillar collagen NC1 domain.
      Contains 1 laminin G-like domain.
    • 结构域The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
    • 翻译后修饰Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      Sulfated on 40% of tyrosines.
    • 细胞定位Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AB collagen antibody
      • Alpha 1 type V collagen antibody
      • Alpha 2 type V collagen antibody
      • CO5A1_HUMAN antibody
      • COL5A1 antibody
      • Col5A2 antibody
      • COL5A2 protein antibody
      • Col5A3 antibody
      • Collagen alpha 1(V) chain antibody
      • Collagen alpha 2 (V) chain precursor antibody
      • Collagen alpha 2(V) chain antibody
      • Collagen alpha 3(V) chain antibody
      • Collagen alpha-1(V) chain antibody
      • Collagen fetal membrane A polypeptide antibody
      • Collagen type V alpha 1 antibody
      • Collagen type V alpha 2 antibody
      • Collagen type V alpha 3 antibody
      • Collagen V alpha 2 polypeptide antibody
      • CollagenV antibody
      • MGC105115 antibody
      • OTTHUMP00000064637 antibody
      • Pro alpha 1 type V collagen antibody
      • Pro alpha 3(V) collagen antibody
      • Procollagen alpha 2(V) antibody
      • Type V preprocollagen alpha 2 chain antibody
      see all

    Anti-Collagen V antibody [1.BB.755] (ab62426)参考文献

    ab62426 has not yet been referenced specifically in any publications.

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