Anti-Collagen Type V抗体[1E2-E4/Col5] (ab112551)

概述

  • 产品名称
    Anti-Collagen Type V抗体[1E2-E4/Col5]
    参阅全部 Collagen Type V 一抗
  • 描述
    小鼠单克隆抗体[1E2-E4/Col5] to Collagen Type V
  • 特异性
    ab112551 has been shown to have no cross-reactivity with type I, III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
  • 经测试应用
    适用于: ELISA, IHC-P, WB, IHC-Frmore details
  • 种属反应性
    与反应: Sheep, Rabbit, Cow, Dog, Human, Pig, Kangaroo
    不与反应: Mouse, Rat, Chicken, Guinea pig
  • 免疫原

    Acid-digested pepsin soluble Dog Type V Collagen.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 97% Phosphate Buffer, 2.9% Sodium chloride
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    1E2-E4/Col5
  • 骨髓瘤
    NS1/1-Ag4-1
  • 同种型
    IgG2a
  • 轻链类型
    kappa

应用

Our Abpromise guarantee covers the use of ab112551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/50.
ab112551 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 184 kDa.
IHC-Fr Use at an assay dependent concentration.

靶标

文献

ab112551 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Pig Tissue lysate - whole (Heart)
Specification
Heart
Treatment
5 pigs are treated with pacemaker for 14 days (pm)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jan 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册