Anti-Collagen Type V抗体[1E2-E4/Col5] (ab112551)

概述

  • 产品名称Anti-Collagen Type V抗体[1E2-E4/Col5]
    参阅全部 Collagen Type V 一抗
  • 描述
    小鼠单克隆抗体[1E2-E4/Col5] to Collagen Type V
  • 特异性ab112551 has been shown to have no cross-reactivity with type I, III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
  • 经测试应用适用于: ELISA, IHC-P, WB, IHC-Frmore details
  • 种属反应性
    与反应: Sheep, Rabbit, Cow, Dog, Human, Pig, Kangaroo
    不与反应: Mouse, Rat, Chicken, Guinea pig
  • 免疫原

    Acid-digested pepsin soluble Dog Type V Collagen.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 97% Phosphate Buffer, 2.9% Sodium chloride
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号1E2-E4/Col5
  • 骨髓瘤NS1/1-Ag4-1
  • 同种型IgG2a
  • 轻链类型kappa

应用

Our Abpromise guarantee covers the use of ab112551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/50.
ab112551 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 184 kDa.
IHC-Fr Use at an assay dependent concentration.

靶标

Anti-Collagen Type V antibody [1E2-E4/Col5] (ab112551)参考文献

ab112551 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Pig Tissue lysate - whole (Heart)
Specification Heart
Treatment 5 pigs are treated with pacemaker for 14 days (pm)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jan 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"