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Full length native protein (purified) (Human).
Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
Our Abpromise guarantee covers the use of ab6311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||1/25 - 1/50. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. PubMed: 18843029
We suggest using an ABC detection system.
|Dot blot||Use at an assay dependent concentration.|
Immunofluorescence analysis of Human endothelial cells, staining Collagen IV with ab6311.
Cells were fixed with formaldehyde and permeabilized with acetone. Samples were incubated with primary antibody (0.5 µg/ml in diluent) for 18 hours at 4°C. An AlexaFluor®488-conjugated goat anti-mouse polyclonal IgG (5 µg/ml) (ab150113) was used as the secondary antibody.
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