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Our Abpromise guarantee covers the use of ab52235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/300 - 1/1000. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).|
ab52235 staining Collagen IV in Human breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). The right image is blocked with the synthesized peptide.
This image is courtesy of an abreview submitted by Dr Guo-Ping Shi from Harvard University.
Blocking: 5% Milk for 16 hours at 4°C.
ab52235 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"