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Full length protein corresponding to Human Collagen IV.
In IHC, recognizes the extracellular – basement membranes.
Our Abpromise guarantee covers the use of ab86042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Use under non reducing condition. Predicted molecular weight: 160 kDa.|
|IHC-P||1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||1/50 - 1/100.|
|ICC||1/50 - 1/100. Methanol fixed.|
|Flow Cyt||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
ab86042 staining Collagen IV in Human kidney tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"