Anti-Collagen III抗体[Col-29] (ab82354)


  • 产品名称Anti-Collagen III抗体[Col-29]
    参阅全部 Collagen III 一抗
  • 描述
    小鼠单克隆抗体[Col-29] to Collagen III
  • 经测试应用适用于: IHC-P, WB, IHC-Frmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Full length protein corresponding to Human Collagen III aa 1-1466.
    Database link: P02461

  • 阳性对照
    • IHC-P: Human skin FFPE tissue sections WB: Human Colo320 Whole cell lysate, HT1080 Whole cell lysate



Our Abpromise guarantee covers the use of ab82354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 138 kDa.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.


  • 功能Collagen type III occurs in most soft connective tissues along with type I collagen.
  • 疾病相关Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
    Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
    Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
  • 序列相似性Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • 翻译后修饰Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CO3A1_HUMAN antibody
    • COL 3A1 antibody
    • COL3A1 antibody
    • Collagen alpha 1(III) chain antibody
    • Collagen alpha-1(III) chain antibody
    • Collagen III alpha 1 chain precursor antibody
    • Collagen III alpha 1 polypeptide antibody
    • Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody
    • Collagen type III alpha 1 antibody
    • Collagen type III alpha antibody
    • EDS4A antibody
    • Ehlers Danlos syndrome type IV, autosomal dominant antibody
    • Fetal collagen antibody
    • Type III collagen antibody
    see all

Anti-Collagen III antibody [Col-29] 图像

  • IHC image of Collagen III staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82354, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Collagen III antibody [Col-29] (ab82354)

    Lane 1 : HT1080 Whole cell lysate
    Lane 2 : Human Colo320 Whole cell lysate

    Predicted band size : 138 kDa

Anti-Collagen III antibody [Col-29] (ab82354)参考文献

This product has been referenced in:
  • Hillestad V  et al. IL-18 neutralization during alveolar hypoxia improves left ventricular diastolic function in mice. Acta Physiol (Oxf) 213:492-504 (2015). Read more (PubMed: 25182570) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Turtle Tissue sections (Skin tumor)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization No
Specification Skin tumor
Blocking step Dako serum-free protein block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative Formaldehyde

Abcam user community

Verified customer

提交于 Jul 03 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 60000µg/mL · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6
Sample Rabbit Tissue sections (Intervertebral Disc (all cells particularly NP))
Specification Intervertebral Disc (all cells particularly NP)
Permeabilization Yes - 1X TBS/0.1% Tween-20
Fixative Formaldehyde

Abcam user community

Verified customer

提交于 May 30 2014

We do not have an order form Lucerna for these antibodies. Plese check with them.


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Ablaufdatum: 31. August 2012

Es freut uns zu hören, dass Sie unser Angebot unsere Antikörper ab23446 und ab82354 inRatteund Schwei...

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Wie versprochen habe ich unsere Kollagen-Antikörper nach den von Ihnen genannten Kriteriengeprüft. Leider habe ich keinen perfekten Treffer gefunden, dafür aber vielleicht ein paar Kandidaten, ...

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I have checked the homology between human and pig for collagen III. It is 93% for the full length protein, therefore there is a high chance of crossreactivity. The following collagen III antibodies were tested in...

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Thank you for your inquiry. The testing laboratory confirmed that the band in WB application is about 70 kDa, because it is run denatured-reduced conditions. If you were to run native, non-reduced conditions, a higher MW would be expected. I hop...

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