概述

  • 产品名称Anti-Collagen III抗体
    参阅全部 Collagen III 一抗
  • 描述
    山羊多克隆抗体to Collagen III
  • 特异性Specificity was ascertained by competition ELISA. Complete inhibition is found if the antibody was preincubated with collagen type III. Inhibition by other types of collagens was observed only at 20-50 times higher concentration. No inhibition was found with fibronectin, fibrinogen and laminin. Characteristic immunostaining pictures of frozen sections of human kidney, liver, skin and heart are produced to certify batch quality.
  • 经测试应用适用于: IHC-Fr, Dot blot, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Extensively purified native collagen type III extracted from human skin.

  • 阳性对照
    • Human kidney, liver, skin and heart for IHC-Fr.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液The antibody was extracted into dilute acidic buffer after mild pepsin digestion.
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Pooled antisera are passed over DEAE cellulose to produce IgG enriched fraction, which is further subjected to absorption with immobilized total human serum proteins in order to remove non specific antibodies. Next, the antisera fraction is absorbed with immunobilized collagen types I, II, IV and V to remove cross reactive antibodies to antigenic determinants common for various collagen types. The affinity purified antibody ab24129 is obtained by binding to immobilized native human collagen type III (the antigen used for immunization), followed by elution with acidic buffer, neutralisation, dialysis, dispensing and lyophilization.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab24129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr
Dot blot
ELISA
  • 应用说明Dot: Use at an assay dependent dilution.
    ELISA: Use at an assay dependent dilution.
    IHC-Fr: Antibodies can be diluted at least 1/20 for immunohistochemical procedures if Peroxidase labeled secondary antibodies are applied. If a FITC labeled secondary antibody is used the antibody can be diluted 1/10.
    Also recommended for use in immunostaining of cultered human cells.
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Collagen type III occurs in most soft connective tissues along with type I collagen.
    • 疾病相关Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
      Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
      Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
    • 序列相似性Belongs to the fibrillar collagen family.
      Contains 1 fibrillar collagen NC1 domain.
      Contains 1 VWFC domain.
    • 翻译后修饰Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
    • 细胞定位Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Alpha 1 type III collagen antibody
      • Alpha1 (III) collagen antibody
      • CO3A1_HUMAN antibody
      • COL 3A1 antibody
      • COL3A1 antibody
      • Collagen alpha 1(III) chain antibody
      • Collagen alpha-1(III) chain antibody
      • Collagen III alpha 1 chain precursor antibody
      • Collagen III alpha 1 polypeptide antibody
      • Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody
      • Collagen type III alpha 1 antibody
      • Collagen type III alpha 1 chain antibody
      • Collagen type III alpha antibody
      • Collagen, fetal antibody
      • EDS4A antibody
      • Ehlers Danlos syndrome type IV, autosomal dominant antibody
      • Fetal collagen antibody
      • Type III collagen antibody
      see all

    Anti-Collagen III antibody (ab24129)参考文献

    This product has been referenced in:
    • Baumann S & Hennet T Collagen Accumulation in Osteosarcoma Cells lacking GLT25D1 Collagen Galactosyltransferase. J Biol Chem 291:18514-24 (2016). Read more (PubMed: 27402836) »

    See 1 Publication for this product

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