Full length native protein (purified) (Dog). Extensively purified native collagen type I and III extracted from dog tail tendon.
Native dog collagen type I and III for dot blotting and ELISA.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: The antibody was extracted into dilute acidic buffer after mild pepsin digestion.
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Pooled antisera are passed over DEAE cellulose to produce IgG enriched fraction. The affinity purified antibody is obtained by binding to immobilized native dog collagen, mixture of type I and III (the antigens used for immunization), followed by elution with acidic buffer, neutralisation, dialysis, dispensing and lyophilization.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution. The antibody can be diluted at least 1/20 for immunohistochemical procedures if Peroxidase labeled secondary antibodies are applied. If a FITC labeled secondary antibody is used the antibody can be diluted 1/10.
WB: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Collagen I is a fibrillar collagen found in most connective tissues, and the only component of the collagen found in cartilage. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with Collagen I. Mutations in this gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.