Anti-Collagen I + Collagen III抗体(ab24135)

概述

  • 产品名称Anti-Collagen I + Collagen III抗体
    参阅全部 Collagen I + Collagen III 一抗
  • 描述
    兔多克隆抗体to Collagen I + Collagen III
  • 特异性Specificity is measured by ELISA using pure pig collagen types I and III. No binding to pig serum proteins is seen at similar dilutions of the antibody. No cross reactivity seen against pig basement membrane collagens type IV and V in immunostaining.
  • 经测试应用适用于: Dot blot, ELISA, IHC-Frmore details
  • 种属反应性
    与反应: Pig
  • 免疫原

    Full length native collagen type I and III from pig skin.

  • 阳性对照
    • Pig kidney and skin. Cultured porcine cells.

性能

  • 形式Lyophilised:Reconstitute with 1ml of distilled water and add preservative if required.
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: None
    Constituents: Whole serum
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Pooled antisera are passed over DEAE-cellulose to produce an IgG enriched fraction. Antibodies specific to type I and type III pig collagen are then immobilised by affinity chromatography and eluted with an acidic buffer. The final purification steps are neutralisation, dialysis and lyophilisation.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab24135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr 1/10 - 1/20.

靶标

  • 相关性Collagen I is a fibrillar collagen found in most connective tissues, and the only component of the collagen found in cartilage. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with Collagen I. Mutations in this gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
  • 细胞定位Secreted
  • 别名
    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • COL3A1 antibody
    • Collagen fetal antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • Collagen type III alpha 1 antibody
    • OI4 antibody
    • Type I collagen alpha 1 chain antibody
    • Type I procollagen antibody
    see all

Anti-Collagen I + Collagen III antibody (ab24135)参考文献

ab24135 has not yet been referenced specifically in any publications.

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That antibody, ab24135, is unlikely to be a good choice, though it is raised against pig collagen. It will be difficult to find anti-collagen antibodies that are reactive with one species and not another, given t...

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